| 用SSR标记法对黑龙江省稻瘟菌无毒基因的检测 |
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| 引用本文： | 张崎峰,靳学慧,蔡鑫鑫,巩双印,刘显元.用SSR标记法对黑龙江省稻瘟菌无毒基因的检测[J].中国农学通报,2010,26(10):250-254. |
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| 作者姓名： | 张崎峰 靳学慧 蔡鑫鑫 巩双印 刘显元 |
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| 作者单位： | 1. 黑龙江省农业科学院黑河分院,黑龙江黑河,164300
2. 黑龙江八一农垦大学,黑龙江大庆,163319 |
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| 基金项目： | 黑龙江省科技攻关项目;黑龙江省农垦总局科技攻关项目 |
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| 摘 要： | 使用与无毒基因Avr-pit、Avr-pia、PRE1紧密连锁的3个SSR标记对黑龙江省不同地区的195个稻瘟菌菌株的DNA进行PCR扩增。结果表明，各无毒基因在黑龙江省的出现频率有明显差异，无毒基因Avr-pit的出现频率为43.59%，无毒基因Avr-pia出现频率36.92%，无毒基因PRE1的出现频率为30.26%。黑龙江省稻瘟病菌的群体结构复杂，在不同地区甚至相同地区的菌株中无毒基因的存在情况都有明显的差异。
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| 关 键 词： | 农业面源污染 农业面源污染 生态补偿 措施和政策 |
| 收稿时间： | 2009-12-03 |
| 修稿时间： | 2010-01-06 |
Identification of Magnaporthe oryzae avirulence gene in Hei Longjiang Province with SSR Marker |
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| Zhang Qifeng,Jin Xuehui,Cai Xinxin,Gong Shuangyin,Liu Xianyuan.Identification of Magnaporthe oryzae avirulence gene in Hei Longjiang Province with SSR Marker[J].Chinese Agricultural Science Bulletin,2010,26(10):250-254. |
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| Authors: | Zhang Qifeng Jin Xuehui Cai Xinxin Gong Shuangyin Liu Xianyuan |
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| Institution: | 1Heihe Branch, Heilongjiang Provincial Academy of Agricultural Sciences, Heihe Heilongjiang 164300;
2Heilongjiang August First Land Reclamation University, Daqing Heilongjiang 163319 |
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| Abstract: | The aim was to study the distribution of Magnaporthe oryzae avirulence gene in Heilongjiang Province. PCR amplification of DNA in 195 Magnaporthe oryzae strains of different region Heilongjiang province by three SSR markers which were closely linked to the avirulence gene Avr-pit, Avr-pia and PRE1. The frequency of different avirulence gene was significant different in Heilongjiang province, and the appearance frequency of these three avirulence genes Avr-pit, Avr-pia and PRE1 were 43.59%, 36.92% and 30.26%. The group structure ofMagnaporthe oryzae was complex in Heilongjiang Province. The distribution of avirulence gene varied in different regions even in the same region. The research laid the foundation for the molecular marker and genetic diversity ofMagnaporthe oryzae and molecular breeding. |
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| Keywords: | SSR markerzz |
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