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| 铜铸熟若蟹中麻痹性毒素成分的分离和鉴定 | |
| 引用本文： | 林华娟,长岛裕二,章超桦,秦小明.铜铸熟若蟹中麻痹性毒素成分的分离和鉴定[J].水产学报,2011,35(10):1540-1546. |
| 作者姓名： | 林华娟 长岛裕二 章超桦 秦小明 |
| 作者单位： | 1. 广东海洋大学食品科技学院,广东湛江524088;水产品深加工广东普通高校重点实验室,广东湛江524088 2. 日本东京海洋大学食品科学技术学部,日本东京,1088477 3. 水产品深加工广东普通高校重点实验室,广东湛江,524088 |
| 基金项目： | 现代农业产业技术体系建设专项(贝类,47) |
| 摘 要： | 在分析了日本珊瑚扇蟹铜铸熟若蟹甲壳组织和内脏组织中毒性含量的基础上,对毒素提取液进行分离纯化,并通过液相色谱法对其毒素种类进行分析。毒性分析结果显示,毒蟹个体的含毒量在37 500~116 000 MU范围,远远超过成人中毒致死量(5 000 MU),但个体间的毒含量有较大差异;毒蟹个体的甲壳组织的比毒性(909~1 610 MU/g)总体高于内脏组织(111~576 MU/g)。Bio-Gel-P2凝胶柱层析纯化结果显示,粗毒素提取液通过反复纯化可以达到纯化目的,毒素的比毒性从4.8 MU/mg上升到30 MU/mg左右;毒素进一步通过Bio-Rex 70离子交换柱层析的分离纯化结果显示,毒素中约有9%为膝沟藻毒素群(GTXs,麻痹性毒素中的一类)。高效液相色谱分析结果显示,膝沟藻毒素群的主要成分为GTX3和GTX2,并含有少量的dcGTX2和dcGTX3。 |
| 关 键 词： | 铜铸熟若蟹 麻痹性毒素(PSP毒素) 膝沟藻毒素(GTXs) 毒性 |
| 收稿时间： | 2010/11/18 0:00:00 |
| 修稿时间： | 7/12/2011 9:58:32 AM |
Isolation and identification of PSP toxin in xanthid crab (Zosimus aeneus)collected from coral reef in Japan |
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| LIN Hua-juan,NAGASHIMA Yuji,ZHANG Chao-hua and QIN Xiao-ming.Isolation and identification of PSP toxin in xanthid crab (Zosimus aeneus)collected from coral reef in Japan[J].Journal of Fisheries of China,2011,35(10):1540-1546. | |
| Authors: | LIN Hua-juan NAGASHIMA Yuji ZHANG Chao-hua and QIN Xiao-ming |
| Institution: | Guangdong Ocean University,Tokyo University of Marine Science and Technology,Guangdong Ocean University,Guangdong Ocean University |
| Abstract: | Some xanthid crabs are known to be toxic and have a serious threat of food poisoning.In present study,the distribution of toxicities in exoskeletal and visceral tissues in the xanthid crab,Zosimus aeneus collected from Ishigaki Island,Okinawa Prefecture,Japan and its paralytic shellfish poisoning toxins(PSP)profiles were examined.Toxicity assay showed that all of six individuals tissue were strongly toxic although there was significant difference among individuals in toxicity.Total toxicity of the individua... |
| Keywords: | xanthid crab(Zosimus aeneus) paralytic shellfish poisoning toxins(PSP) gonyatoxins(GTXs) toxicity |
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