Evaluation of Activator Solutions, Motility Duration, and Short-Term Storage of Paddlefish Spermatozoa |
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| Authors: | Steven D Mims |
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| Institution: | Aquaculture Research Center, Community Research Service, Kentucky State University, Frankfort, Kentucky 40601 USA |
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| Abstract: | Milt from paddlefish ( Polyodon sparhula ) was collected, examined for motility, and pooled. Six different solutions (dechlorinated tap water, ovarian fluid, bacteriostatic water, distilled water, 0.9% sodium chloride saline water, and milt plasma) were examined for activation and duration of spermatozoal motility. Most solutions except saline solution and milt plasma promoted activation of spermatozoa. Dechlorinated tap water was found to be the best activator with significantly longer duration of spermatozoal motility than the other tested solutions. Saline solution and milt plasma appear to be useful as carrier solutions because of their property of maintaining inactive, viable spermatozoa.
Paddlefish milt in three treatments was evaluated for chilled storage without additives (MF); with 500 IU penicillin and 500 mg streptomycin mix in milt plasma (MP); and 0.9% saline solution (MS)]. The three treatments were stored at 2.4 ± 0.9 C in a precooled, moisture-saturated oxygen environment and observed every 24 h for 9 d. Motility of activated spermatozoa in MS (7 d) was significantly longer than MF or MP treatments (7 d). Spermatozoa in MS had the longest chilled storage time (8 d). |
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