| A型肉毒毒素重链C片段基因的克隆及序列分析 |
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| 引用本文： | 于师宇,孟宪梅,潘风光,任洪林,柳增善.A型肉毒毒素重链C片段基因的克隆及序列分析[J].延边大学农学学报,2005,27(1):10-13. |
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| 作者姓名： | 于师宇 孟宪梅 潘风光 任洪林 柳增善 |
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| 作者单位： | 解放军军需大学,军事兽医系,吉林,长春,130062 |
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| 基金项目： | 国家自然科学基金资助项目(30371059) |
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| 摘 要： | 成功克隆了A型肉毒毒素重链C片段部分基因．以肉毒梭菌(62A)基因组DNA为模板，以此基因特异性引物为介导，采用Touchdown PCR方法，从肉毒梭菌基因组中扩增出目的基因片段后，将其克隆入T载体，进行酶切鉴定和序列分析．结果表明，此基因片段与(Genbank中的BoNTa基因(收录号：M30196)核苷酸序列的同源性为100％，说明目的基因得到了成功地克隆，为后续研究奠定了基础．
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| 关 键 词： | A型肉毒毒素 重链 克隆 基因 肉毒梭菌 序列分析 明目 特异性引物 酶切 同源性 |
| 文章编号： | 1004-7999(2005)01-0010-04 |
| 修稿时间： | 2005年2月25日 |
Cloning and sequence analysis of heavy chain C fragment of Botulinum Neurotoxin Serotype A |
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| YU Shi-yu,MENG Xian-mei,PAN Feng-guang,REN Hong-lin,LIU Zeng-shan.Cloning and sequence analysis of heavy chain C fragment of Botulinum Neurotoxin Serotype A[J].Journal of Agricultural Science Yanbian University,2005,27(1):10-13. |
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| Authors: | YU Shi-yu MENG Xian-mei PAN Feng-guang REN Hong-lin LIU Zeng-shan |
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| Institution: | YU Shi-yu,MENG Xian-mei,PAN Feng-guang,REN Hong-lin,LIU Zeng-shan~* |
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| Abstract: | Part genes of BoNTaHc were cloned successfully. We used the genomic DNA of BoNTa (62A) as model, mediated the specific primer, amplified the purpose gene fragment from BoNTa with the Touchdown PCR technique and cloned it into T Vector. The identification of digestion and sequence analysis showed that the homology of nucleotide sequences between the genomic fragment and BoNTa gene (Accession Number:M30196) in GenBank was 100% which proved that the purpose gene was cloned successfully and made the foundation for the further research. |
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| Keywords: | BoNTaHc gene clone PCR gene sequence analysis |
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