| 牙鲆EST资源的SSR信息分析 |
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| 引用本文： | 陈松波,龚丽,刘海金.牙鲆EST资源的SSR信息分析[J].东北农业大学学报,2010,41(10). |
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| 作者姓名： | 陈松波 龚丽 刘海金 |
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| 基金项目： | 国家科技支撑计划，国家鲆鲽类产业技术体系，公益性农业科研专项 |
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| 摘 要： | 应用生物信息学方法,对牙鲆EST-SSRs的分布频率及碱基重复特点进行了归纳与分析。结果表明,5 927条牙鲆非冗余EST序列通过在线搜索共检索出471个SSRs,分布于390条EST中,出现频率为7.95%,平均分布距离为7.9 kb。包括了112种重复基元,其中二核苷酸重复基元占主导地位,占总SSR的59.02%。AC是二核苷酸中的优势基元,占二核苷酸重复类型的16.91%。三核苷酸重复基元、四核苷酸重复基元和六核苷酸重复基元分布比较分散,三核苷酸重复基元中GAG数量最多,但仅占三核苷酸重复类型的7.26%。研究结果为牙鲆EST-SSR标记的开发及应用奠定了理论基础。
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| 关 键 词： | 牙鲆 EST SSR信息 EST-SSR 重复基元 |
Analysis of SSR information in EST resources of Japanese flounder (Paralichthys olivaceus) |
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| CHEN Songbo,GONG Li,LIU Haijin.Analysis of SSR information in EST resources of Japanese flounder (Paralichthys olivaceus)[J].Journal of Northeast Agricultural University,2010,41(10). |
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| Authors: | CHEN Songbo GONG Li LIU Haijin |
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| Abstract: | By applying bioinformatics methods,the distribution frequency and characteristic of base repeat of EST-SSRs were induced and analyzed in Japanese flounder(Paralichthys olivaceus).The results showed that 5 927 non-redundant ESTs were screened and 471 SSRs were mined out.The frequency of these EST-SSRs was 7.95% and the mean distance of distribution was 7.9 kb in non-redundant ESTs.All these SSRs included 112 kinds of repeat motifs.Among them the dinucleotide repest motif was the dominant type(59.02%),the trinucleotide repeat motif took the second place(26.33%).AC was the most common motif in dinucleotide,accounting for 16.91%.The distribution of trinucleotide,tetranucleotide and hexanucleotide repeats were dispersive.The dominant motif was GAG in trinucleotide repeats,only accounting for 7.26% in all the trinucleotide repeats.All these established the theoretic foundation for development and application of EST-SSR in Japanese flounder. |
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| Keywords: | Japanese flounder(Paralichthys olivaceus) EST SSR information EST-SSR repeat motif |
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