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Transmission of potato leafroll virus to or from tuber slices by aphids feeding through a membrane
Authors:J Syller  D Peters
Institution:(1) Department of Virology, Agricultural University, POB 8045, 6700 EM, Wageningen, the Netherlands;(2) Present address: Institute for Potato Research, Młochów, 05-832, Rozalin, Poland
Abstract:Summary Eye-bearing slices, cut from healthy potato tubers and placed between Parafilm membranes, were inoculated with potato leafroll virus (PLRV) byMyzus persicae. PLRV was detected by ELISA and by transmission tests in tuber slices and in plants grown from the slices of the susceptible cv. Désirée, but not in those of the resistant cv. Arkula. These results suggest that PLRV replication and transport within tuber phloem is controlled by specific mechanisms of resistance.M. persicae was also able to acquire and transmit PLRV toPl floridana from slices cut from tubers of infected plants. The aphids effectively transmitted PLRV from slices cut from the sprouting rose end but they failed to transmit it from slices cut from the heel end of tubers.
Keywords:resistance  inoculation of tuber slices  infection of plants
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