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Effect of the type of extraction solution and length of storage on the distribution of lactate dehydrogenase isoenzymes in piglet liver homogenate |
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| Authors: | U Schuhmacher E Grün |
| Abstract: | Type of extraction solution applied to preparation of homogenate of piglet liver is of negligible importance, if immediately follows electrophoretic separation of tissue material. --Storage of liver homogenates in frozen state over 30 days involves insignificant changes of distribution of LD isoenzymes in 0.25 M sucrose, distinct changes in 0.1 M Tris-buffer, pH 7,5, and important ones in mercaptoaethanol-containing extraction solution, applied to preparation of tissue homogenates. |
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