|
|||||
|
|
| 中国七省(自治区)马铃薯黄萎病病情及优势病原菌致病力分析 | |
| 引用本文： | 李社增,周洪友,鹿秀云,年冠臻,郭庆港,赵卫松,东保柱,申建芳,王培培,张晓云,闫磊,马平.中国七省(自治区)马铃薯黄萎病病情及优势病原菌致病力分析[J].植物病理学报,2018,48(5):656-665. |
| 作者姓名： | 李社增 周洪友 鹿秀云 年冠臻 郭庆港 赵卫松 东保柱 申建芳 王培培 张晓云 闫磊 马平 |
| 作者单位： | 河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室,保定071000; 内蒙古农业大学,呼和浩特010019 |
| 基金项目： | 公益性行业(农业)科研专项经费(201503109) |
| 摘 要： | 2013-2016年,对我国内蒙古、河北、甘肃、黑龙江、山西、宁夏和云南7省(自治区)53县市245块马铃薯种植田黄萎病的发生、病样采集和病原分离开展了研究工作,通过分子生物学方法鉴定了病原菌分类地位,并通过温室试验研究了主要病原菌大丽轮枝菌的致病力分化情况。结果表明:(1)我国北方6省(自治区)内蒙古、河北、甘肃、黑龙江、山西和宁夏属于马铃薯黄萎病病区,云南省属于无病区;(2)引起我国马铃薯黄萎病的病原菌为大丽轮枝菌和黑白轮枝菌两种,占比分别为75.5%和24.5%,大丽轮枝菌为相对优势病原菌种类;(3)根据病原菌种类可将我国北方6省(自治区)病田划分为大丽轮枝菌病田、黑白轮枝菌病田及两菌混生病田。其中甘肃为大丽轮枝菌病田,宁夏为混生病田,山西为大丽轮枝菌病田和混生病田,内蒙古、河北和黑龙江3种病田均存在。两菌混生病田中各类病原菌属于单独侵染致病。(4)聚类分析表明来自甘肃、河北和内蒙古3省(自治区)马铃薯的82个大丽轮枝菌菌株在马铃薯上的致病力至少可以分为3个聚类组,相应菌株的病害发展曲线下面积(AUDPC)均值聚类组间存在显著差异,相应可分为强、中、弱3个致病力类型,并确定了相应95%置信区间,其中强致病力菌株在3省(自治区)供试病原菌中所占比例分别为3.33%、21.74%和10.34%。 |
| 关 键 词： | 马铃薯黄萎病 发生 大丽轮枝菌 黑白轮枝菌 致病力 |
| 收稿时间： | 2017-11-24 |
Occurrence of potato Verticillium wilt and analysis of pathogenicity differentiation of the major pathogen in seven provinces of China |
|
| LI Shezeng,ZHOU Hongyou,LU Xiuyun,NIAN Guanzhen,GUO Qinggang,ZHAO Weisong,DONG Baozhu,SHEN Jianfang,WANG Peipei,ZHANG Xiaoyun,YAN Lei,MA Ping.Occurrence of potato Verticillium wilt and analysis of pathogenicity differentiation of the major pathogen in seven provinces of China[J].Acta Phytopathologica Sinica,2018,48(5):656-665. | |
| Authors: | LI Shezeng ZHOU Hongyou LU Xiuyun NIAN Guanzhen GUO Qinggang ZHAO Weisong DONG Baozhu SHEN Jianfang WANG Peipei ZHANG Xiaoyun YAN Lei MA Ping |
| Institution: | Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China; Inner Mongolia Agricultural University, Hohhot 010019, China |
| Abstract: | Survey for occurrence of potato Verticillium wilt (PVW), collection of the diseased samples and pathogen isolation were carried out in 245 potato production fields distributed in 53 counties of 7 provinces in China from 2013 to 2016. Molecular and biological methods was employed for the classification of the pathogens and the pathogenicity differentiation of Verticillium dahliae causing PVW was evaluated in greenhouse. Results indicated that PVW was found throughout the 6 provinces in northern China covering Inner Mongolia, Hebei, Gansu, Heilongjiang, Shanxi and Ningxia, but not in Yunnan province. The pathogen causing PVW was identified as V. dahliae (Vd) and V. albo-atrum (Vaa), and V. dahliae was the major pathogen with isolation proportion of 75.5%. The diseased fields over the 6 provinces in northern China could be divided into 3 different types including Vd-only, Vaa-only and Mixed pathogens field based on the distribution of the pathogens. The Vd-only pathogen field was only found in Gansu, the Mixed pathogens field only in Ningxia, Vd-only and Mixed pathogens field in Shanxi, and 3 types diseased fields were found in Inner Mongolia, Hebei and Heilongjiang province. All kinds of pathogens in the Mixed pathogens field belonged to individual infection. Clustering analysis of the pathogen’s pathogenicity was carried out by using 82 isolates of V. dahliae from Gansu, Hebei and Inner Mongolia. The result showed that all tested pathogen isolates could be clustered into at least 3 groups, and the AUDPC mean corresponding to the different cluster groups showed significant differences at P<0.05. In addition, the isolates could be divided into 3 pathogenic types of strong, weak and moderate, while the 95% confidence intervals were calculated correspondingly. Among them, the proportion of strong pathogenic isolates in 3 provinces was 3.33%, 21.74% and 10.34%, respectively. |
| Keywords: | potatoVerticilliumwilt occurrence Verticilliumdahliae Verticilliumalbo-atrum pathogenicity |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《植物病理学报》浏览原始摘要信息 | |
| 点击此处可从《植物病理学报》下载免费的PDF全文 | |