| 枯草芽胞杆菌YB-05对小麦全蚀病菌胞内酶活的影响 |
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| 引用本文： | 孙润红,徐俊蕾,杨丽荣,张洁,夏明聪,武超,薛保国,吴坤.枯草芽胞杆菌YB-05对小麦全蚀病菌胞内酶活的影响[J].中国生物防治学报,2018,34(1):156-162. |
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| 作者姓名： | 孙润红 徐俊蕾 杨丽荣 张洁 夏明聪 武超 薛保国 吴坤 |
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| 作者单位： | 1. 河南省农业科学院植物保护研究所/河南省农作物病虫害防治重点实验室/农业部华北南部农作物有害生物综合治理重点实验室/河南省作物保护国际联合实验室, 郑州 450002;2. 河南农业大学生命学院, 郑州 450002 |
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| 基金项目： | 河南省农业科学院科研发展专项资金（201513117）；河南省博士后基金（2015107）；小麦主要病虫害绿色防控产品的研发与产业化（141100111100）；河南省基础与前沿基金（162300410156） |
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| 摘 要： | 小麦全蚀病菌是影响小麦产量和质量的主要致病菌之一，前期研究表明枯草芽胞杆菌YB-05对小麦全蚀病菌的生长具有抑制作用。本试验拟通过研究该菌对小麦全蚀病菌相关酶系的诱导变化情况，解析其对小麦全蚀病菌的抑制作用机理。本试验以小麦全蚀病菌Gaeumannomyces gramini（Sacc.）Arx et Oliver var. tritici（Sacc.）Walker为靶标菌，加入终浓度为MIC50枯草芽胞杆菌YB-05发酵液，通过显微镜观察菌株YB-05发酵液对小麦全蚀病菌菌丝结构和形态的影响，通过酶活力测定检测菌株YB-05发酵液对小麦全蚀病菌胞内苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、过氧化氢酶（CAT）、多酚氧化酶（PPO）和超氧化物歧化酶（SOD）防御酶活的影响，同时检测菌株YB-05发酵液在离体/活体条件下对小麦全蚀病菌线粒体复合酶Ⅱ/Ⅲ活力的影响。小麦全蚀病菌经菌株YB-05发酵液处理后，显微镜观察到其菌丝变粗、断裂，顶端膨大，分枝增多；处理8 d后，胞内的PAL、POD、CAT、PPO和SOD活性比对照依次高24.52%、72.67%、81.81%、80.36%和112.48%；离体条件处理，线粒体复合酶Ⅱ和Ⅲ的活性与对照组相比差异不显著，而活体条件处理下差异显著，线粒体复合酶Ⅱ和Ⅲ分别比对照组分别降低43.95%和55.87%。菌株YB-05发酵液通过结合小麦全蚀病菌线粒体复合酶Ⅱ和Ⅲ的相关基因，从而影响该基因的表达，抑制小麦全蚀病菌线粒体复合酶Ⅱ和Ⅲ的活力，影响小麦全蚀病菌的呼吸，进而导致抑制小麦全蚀病菌菌丝畸变，从而影响小麦全蚀病菌的正常生长。
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| 关 键 词： | 枯草芽胞杆菌 发酵液 小麦全蚀病菌 防御酶活性 线粒体复合酶Ⅱ和Ⅲ酶活性 |
| 收稿时间： | 2017-06-26 |
Effect of Bacillus subtilis YB-05 Fermentation Broth on Gaeumannomyces gramini Enzyme Activities |
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| SUN Runhong,XU Junlei,YANG Lirong,ZHANG Jie,XIA Mingcong,WU Chao,XUE Baoguo,WU kun.Effect of Bacillus subtilis YB-05 Fermentation Broth on Gaeumannomyces gramini Enzyme Activities[J].Chinese Journal of Biological Control,2018,34(1):156-162. |
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| Authors: | SUN Runhong XU Junlei YANG Lirong ZHANG Jie XIA Mingcong WU Chao XUE Baoguo WU kun |
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| Institution: | 1. Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China/International Joint Research Laboratory for Crop Protection of Henan/Biological Pesticides Engineering Research Center of Henan Province/Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;2. College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | Wheat take-all pathogen is one of the main pathogenic funguses which exert influences on yield and quality of wheat, there have previous studies to prove that biocontrol strain B. subtilis YB-05 inhibits the growth of wheat take-all pathogen. This study intended to study the changes of the related enzymes in wheat take-all pathogen which were induced by B. subtilis YB-05, and explored the inhibition mechanism of B. subtilis YB-05 fermentation broth against wheat take-all pathogen. Wheat take-all pathogen was treated with B. subtilis YB-05 fermentation broth which the concentration reached MIC50, the effects of B. subtilis YB-05 fermentation broth on mycelium structure and morphology of wheat take-all pathogen were observed by microscope; CAT, POD, PPO, SOD and PAL activity of wheat take-all pathogen were detected through enzyme activity assay, at the same time the enzymatic activities of mitochondrial enzyme complexes Ⅱ and Ⅲ of wheat take-all pathogen in vitro/vivo were studied. The results showed that B. subtilis YB-05 fermentation broth resulted in aberrant growth of wheat take-all pathogen; The enzymatic activities of PAL, POD, CAT, PPO and SOD in wheat take-all pathogen treated with B. subtilis YB-05 fermentation broth for 8 day were 24.52%, 72.67%, 81.81%, 80.36% and 112.48%, lower than the control group, respectively; The enzymatic activities of mitochondrial enzyme complexes Ⅱ and Ⅲ were not changed significantly with the control in vitro. However, the enzymatic activities of mitochondrial enzyme complexes Ⅱ and Ⅲ were lower 43.95% and 55.87% than the control group in vivo, respectively. B. subtilis YB-05 fermentation broth affected the gene expression by combining the related genes of mitochondrial enzyme complexes Ⅱ and Ⅲ in wheat take-all pathogen, and then reduced the respiratory metabolism of wheat take-all pathogen by inhibiting the mitochondrial enzyme complexes Ⅱ and Ⅲ enzymatic activity, and finally caused the growth inhibition. |
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| Keywords: | Bacillus subtilis fermentation broth wheat take-all pathogen activities of defensive enzyme mitochondrial enzyme complexes Ⅱ and Ⅲ enzymatic activity |
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