| 高原草地杀蝗菌株嗜烟碱节杆菌Arthrobacter nicotinovorans的分离鉴定及发酵条件优化 |
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| 引用本文： | 刘英,岳莹,查艳梅,金洪,侯太平,陶科.高原草地杀蝗菌株嗜烟碱节杆菌Arthrobacter nicotinovorans的分离鉴定及发酵条件优化[J].中国生物防治学报,2018,34(5):708-714. |
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| 作者姓名： | 刘英 岳莹 查艳梅 金洪 侯太平 陶科 |
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| 作者单位： | 四川大学生命科学学院/生物资源与生态环境教育部重点实验室, 成都 610064 |
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| 基金项目： | 国家重点科研计划（2016YFC0502004） |
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| 摘 要： | 从青藏高原采集的土壤中筛选到一株具有杀蝗虫活性的细菌，根据菌株形态和生理生化特征，结合16S rRNA序列分析，鉴定为嗜烟碱节杆菌Arthrobacter nicotinovorans，编号GZ1-6。室内测定了菌株GZ1-6对东亚飞蝗若虫的致病力，结果显示菌株GZ1-6菌悬液对若虫的校正死亡率达到75.56%。测定了碳氮源、温度、初始pH、装液量以及培养时间对菌株GZ1-6生长和杀虫活性的影响，得到菌株GZ1-6分别在以蔗糖和酵母浸膏为碳氮源，pH 7.5、恒温35℃的条件下生长最好，培养2 d，杀蝗活性较优化前提高了7.77%。菌株GZ1-6能够有效侵染东亚飞蝗并将其致死，可作为优良菌株进行杀蝗虫生物农药的进一步研发。
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| 关 键 词： | 分离鉴定 嗜烟碱节杆菌 东亚飞蝗 毒力测定 发酵条件优化 |
| 收稿时间： | 2018-02-05 |
Isolation and Identification for Arthrobacter nicotinovorans against Locust from Qinghai-Tibetan Plateau and Optimization of Culture Conditions |
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| LIU Ying,YUE Ying,ZHA Yanmei,JIN Hong,HOU Taiping,TAO Ke.Isolation and Identification for Arthrobacter nicotinovorans against Locust from Qinghai-Tibetan Plateau and Optimization of Culture Conditions[J].Chinese Journal of Biological Control,2018,34(5):708-714. |
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| Authors: | LIU Ying YUE Ying ZHA Yanmei JIN Hong HOU Taiping TAO Ke |
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| Institution: | Key Laboratory of Bio-resource and Eco-environment, Ministry of Education/College of Life Sciences, Sichuan University, Chengdu 610064, China |
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| Abstract: | A bacteria strain selected from the soil of Qinghai-Tibetan Plateau was identified as Arthrobacter nicotinovorans and named GZ1-6 based on the 16S rRNA sequence analysis, physiological and biochemical characteristics. Virulence of GZ1-6 to nymph of Locusta migratoria manilensis was evaluated in the laboratory, with a corrected accumulative mortality over 75.56%. The optimum fermentation conditions were determined to be temperature 35℃, pH 7.5, sucrose and yeast extract as carbon and nitrogen source, respectively. The optimized fermentation products showed a 7.7% higher toxicity against L. migratoria manilensis compared with pre-optimum. The results indicate that the strain GZ1-6 can be used as a potential biocontrol agent against the locust in the field. |
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| Keywords: | isolation and identification Arthrobacter nicotinovorans Locusta migratoria manilensis toxicity test optimized fermentation condition |
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