| 烟草疫霉SSR分子标记的开发与初步应用 |
| |
| 引用本文： | 崔林开,高鹏飞,刘精精,康业斌,胡艳红,赵启美.烟草疫霉SSR分子标记的开发与初步应用[J].植物病理学报,2018,48(4):474-481. |
| |
| 作者姓名： | 崔林开 高鹏飞 刘精精 康业斌 胡艳红 赵启美 |
| |
| 作者单位： | 河南科技大学林学院,洛阳 471023 |
| |
| 基金项目： | 公益性行业(农业)科研专项(201303018);国家自然科学基金(U1404318);河南科技大学培育基金(2013ZCX013);河南科技大学青年基金(2012QN001) |
| |
| 摘 要： | 为开发烟草疫霉的SSR分子标记,利用MISA软件搜索烟草疫霉基因组序列中的 SSR位点,共发现1 311个SSR位点,优势SSR位点为二核苷酸和三核苷酸,分别占总 SSR 位点的56.45%和39.36%;根据分析到的SSR位点使用Primer 5.0软件设计48对SSR引物,以7株烟草疫霉的DNA 为模板对这些引物进行筛选,共获得扩增条带清晰且具有多态性的SSR引物20对,然后使用其中的6对SSR对32株烟草疫霉进行UPGMA聚类分析,遗传相似系数在 0.60~1.00 之间,在相似系数0.70水平上,可将其划分为3个类群,类群I包含29个菌株,类群II包含1个菌株,类群III包含2个菌株,显示出较低的遗传分化水平。这些SSR引物的开发将为研究我国烟草疫霉的遗传多样性、群体遗传结构以及遗传图谱构建等奠定基础。
|
| 关 键 词： | 烟草疫霉 SSR 筛选 聚类分析 |
| 收稿时间： | 2017-10-18 |
Development of SSR markers for Phytophthora nicotianae and its preliminary application |
| |
| CUI Lin-kai,GAO Peng-fei,LIU Jing-jing,KANG Ye-bin,HU Yan-hong,ZHAO Qi-mei.Development of SSR markers for Phytophthora nicotianae and its preliminary application[J].Acta Phytopathologica Sinica,2018,48(4):474-481. |
| |
| Authors: | CUI Lin-kai GAO Peng-fei LIU Jing-jing KANG Ye-bin HU Yan-hong ZHAO Qi-mei |
| |
| Institution: | Forestry College, Henan University of Science and Technology, Luoyang 471023, China |
| |
| Abstract: | The objective of this research is to develop SSR markers in Phytophthora nicotianae. A total of 1 311 SSR loci were identified from the P. nicotianae genome using MISA software. Trinucleotide and tetranucleotide were dominant motifs which accounted for 56.45% and 39.36%, respectively. Based on identified SSR loci, 48 pairs of primers were designed using Primer 5.0 software. DNAs of seven P. nicotianae isolates were used as template in order to screen these primers. Twenty pairs of primers of which amplified bands were clear and polymorphic were identified. Based on amplification results of six pairs of primers, the UPGMA dendrogram of 32 isolates illustrated that they were separated clearly into three groups at 0.70 genetic similarity. Group I inclu-ded 29 isolates. Group II included one isolate and Group III included two isolates. The genetic similarity coefficient of 32 isolates varied from 0.60 to 1.00. Genetic differentiation among isolates was low. Development of these SSR markers laid foundation for studying genetic diversity, population genetic structure, and linkage map construction of P. nicotianae. |
| |
| Keywords: | Phytophthora nicotianae simple sequence repeats screening clustering analysis |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
