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| 水稻稻粒黑粉病病原菌鉴定及致病性测定 | |
| 引用本文： | 王爱军,江波,富蓉,王娜,殷得所,李平,张靖,郑爱萍.水稻稻粒黑粉病病原菌鉴定及致病性测定[J].植物病理学报,2018,48(3):297-304. |
| 作者姓名： | 王爱军 江波 富蓉 王娜 殷得所 李平 张靖 郑爱萍 |
| 作者单位： | 四川农业大学水稻研究所,成都611130; 四川农业大学西南作物基因资源与遗传改良教育部重点实验室,雅安 625014; 长江师范学院生命科学与技术学院,重庆408100; 湖北省农业科学院粮食作物研究所,武汉430064 |
| 基金项目： | 重庆市教育委员会科学技术研究项目(KJ15012017) |
| 摘 要： | 为明确我国南方杂交水稻制繁种区稻粒黑粉病病原菌及其致病力情况,于2015年8月从安徽、湖南、江苏、贵州、四川遂宁、四川新津、四川温江7个主要杂交稻制繁种区采集稻粒黑粉病样本,采用冬孢子悬浮液分离法进行病原菌的分离。基于形态学、菌丝细胞核荧光染色和r DNA-ITS序列分析对病原菌进行鉴定,并应用柯赫氏法则对不同地区病样中分离的纯培养物致病性进行测定。结果表明分离到的35株病原菌菌株并无明显形态学差异,冬孢子呈球形或椭圆形,次生小孢子单细胞核,呈线状或弯曲状两种类型,与已报道的黑粉菌属Tilletia horrida形态特征相似;r DNA-ITS序列分析表明,选取的7株病原菌菌株与T.horrida DQ827699同源性达100%,说明所分离菌株为稻粒黑粉病菌;致病性试验表明,7株病原菌菌株侵染水稻稻粒均能产生稻粒黑粉病的典型症状,其中分离自江苏的菌株JS造成的单穗病粒数和产量损失最大,致病力较强,且萌芽率最高,分离自不同地区的菌株致病力具有明显差异。该7株病原菌最适生长温度为28℃,光照对其生长没有明显影响。 |
| 关 键 词： | 水稻 稻粒黑粉病菌 鉴定 致病性 |
Identification and pathogenicity of pathogen causing kernel smut on rice |
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| WANG Ai-jun,JIANG Bo,FU Rong,WANG Na,YIN De-suo,LI Ping,ZHANG Jing,ZHENG Ai-ping.Identification and pathogenicity of pathogen causing kernel smut on rice[J].Acta Phytopathologica Sinica,2018,48(3):297-304. | |
| Authors: | WANG Ai-jun JIANG Bo FU Rong WANG Na YIN De-suo LI Ping ZHANG Jing ZHENG Ai-ping |
| Institution: | Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Southwest Crop Gene Resource & Genetic Improvement of Ministry of Education, Sichuan Agricultural University, Ya′an 625014, China; College of Life Science and Technology,Yangtze Normal University,Chongqing 408100,China; Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China |
| Abstract: | In order to confirm the pathogen of kernel smut on rice seeds and its pathogenicity in the hybrid rice seed production areas of south China, the isolates were obtained from the samples of rice seeds with kernel smut, collected from Anhui, Hunan, Jiangsu, Guizhou, Suining, Xinjin, and Wenjiang in August 2015, by culturing spore suspension. The following characterizations were conducted according to the morphological characteristics, nucleus fluorescence staining, and rDNA-ITS sequence analysis. Meanwhile, the pathogenicity test of the pure cultures from the infected rice tissues was performed according to Koch’s postulates. The results showed that the thirty-five strains exhibited no significant differences in morphology. The teliospores in shape was sphere or near round, and the secondary microspore were liner, curve and mononucleate. The results of rDNA-ITS sequence analysis showed that 7 isolates were 100% identity with Tilletia horrida DQ827699. The pathogenicity tests showed that all the strains could cause the typical symptoms on rice seed of rice kernel smut with different level of pathogenicity, in which the JS strain from Jiangsu reached the highest level of pathogenicity and conidial germination rate leading to the highest losses in yield and amount of infected seeds per spike. The optimal temperature for mycelial growth of the seven test strains were 28℃ while light did not obviously affect mycelial growth. |
| Keywords: | rice Tilletia horrida identification pathogenicity |
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