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| 线粒体羧酸盐转运蛋白BcMito-TTP参与灰葡萄孢致病、产孢及菌核发育的研究 | |
| 引用本文： | 梁克力,刘四,程家森,谢甲涛,付艳苹.线粒体羧酸盐转运蛋白BcMito-TTP参与灰葡萄孢致病、产孢及菌核发育的研究[J].植物病理学报,2018,48(1):80-88. |
| 作者姓名： | 梁克力 刘四 程家森 谢甲涛 付艳苹 |
| 作者单位： | 湖北省作物病害检测和安全控制重点实验室, 华中农业大学, 武汉 430070 农业微生物学国家重点实验室, 华中农业大学, 武汉430070 |
| 基金项目： | 国家自然科学基金(31371895);公益性行业(农业)科技专项(201303025) |
| 摘 要： | 灰葡萄孢菌(Botrytis cinerea)可侵染1 400多种植物,给全世界农作物生产造成了严重的损害。BcMito-TTP蛋白是一种进化保守的线粒体羧酸盐转运蛋白,酿酒酵母中研究发现该羧酸盐转运蛋白主要负责催化柠檬酸盐通过线粒体内膜流出以交换羧酸盐H+离子等。本研究通过对BcMito-TTP基因进行敲除和功能互补发现,与原始菌株相比,敲除转化子生长速度没有差异,但产孢量有所降低,致病力明显减弱,菌核形成进程推迟。BcMito-TTP敲除转化子在添加CH3COONa培养基中生长速度变慢且将BcMito-TTP基因互补可以恢复上述生物学性状。推测BcMito-TTP蛋白的缺失阻碍了CH3COONa在灰葡萄孢体内的运输继而影响其孢子和菌核的形成,BcMito-TTP 基因参与灰葡萄孢致病力分子机制正在进一步研究之中。 |
| 关 键 词： | 灰葡萄孢 线粒体羧酸盐转运蛋白 孢子产量 致病力 菌核发育 |
| 收稿时间： | 2017-05-12 |
The mitochondrial tricarboxylate transport protein BcMito-TTP is involved in conidial production,pathogenicity and sclerotial development of Botrytis cinerea |
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| LIANG Ke-li,LIU Si,CHENG Jia-sen,XIE Jia-tao,FU Yan-ping.The mitochondrial tricarboxylate transport protein BcMito-TTP is involved in conidial production,pathogenicity and sclerotial development of Botrytis cinerea[J].Acta Phytopathologica Sinica,2018,48(1):80-88. | |
| Authors: | LIANG Ke-li LIU Si CHENG Jia-sen XIE Jia-tao FU Yan-ping |
| Institution: | Provincial Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China |
| Abstract: | Botrytis cinerea can infect more than 1 400 species of plants and cause serious losses around the world. Mito-TTP is a class of conserved proteins that are mainly responsible for transferring tricarboxylate substances in or out of mitochondria. It catalyzes obligatory exchanges of the dibasic form of a tricarboxylic acid for another tricarboxylate/H+, a dicarboxylate or phosphoenolpyruvate in Saccharomyces cerevisiae. In this research, the Mito-TTP gene was disrupted to study its biological roles in B. cinerea. The resulting mutants showed no difference in fungal growth compared to the strain B05.10, but displayed a number of phenotypic changes including reduced levels of conidial production and fungal virulence, the delayed development of sclerotia, and the increased sensitivity to CH3COONa supplemented in growth media. All of these phenotypes could be rescued by complementing the mutants with the BcMito-TTP gene. The results suggested that BcMito-TTP is involved in the process of conidial production, sclerotial development, and the virulence of pathogen. The mechanisms are still under investigation. |
| Keywords: | Botrytis cinerea mitochondrial tricarboxylate transport protein conidial production virulence sclerotial development |
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