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| 四川省17个主栽小麦品种中Lr26和Lr19检测 | |
| 引用本文： | 高璞,周悦,郭聪,赵卿颖,张培培,刘贺星,李在峰,刘大群.四川省17个主栽小麦品种中Lr26和Lr19检测[J].植物病理学报,2018,48(2):275-279. |
| 作者姓名： | 高璞 周悦 郭聪 赵卿颖 张培培 刘贺星 李在峰 刘大群 |
| 作者单位： | 河北农业大学植物保护学院,保定071001; 保定学院生物系,保定071000 |
| 基金项目： | 国家自然科学基金(31361140367、31571662、31601299);河北省自然科学基金(C2016104013) |
| 摘 要： | 正小麦叶锈病是一种重要的小麦病害,培育和种植抗病品种是最为经济有效的防治方法。我国在小麦抗病育种工作中引入了1BL/1RS易位系,已知小麦抗病基因Lr26、Pm8、Yr9和Sr31均位于1BL/1RS易位系上~(1])。然而目前Lr26已丧失抗性~(2]),所以育种过程中需要谨慎考虑Lr26的使用。Lr19来源于长穗偃麦草~(3]),在我国目前为有效的 |
Detection of leaf rust resistance genes Lr26 and Lr19 in 17 commercial wheat cultivars from Sichuan Province |
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| GAO Pu,ZHOU Yue,GUO Cong,ZHAO Qing-ying,ZHANG Pei-pei,LIU He-xing,LI Zai-feng,LIU Da-qun.Detection of leaf rust resistance genes Lr26 and Lr19 in 17 commercial wheat cultivars from Sichuan Province[J].Acta Phytopathologica Sinica,2018,48(2):275-279. | |
| Authors: | GAO Pu ZHOU Yue GUO Cong ZHAO Qing-ying ZHANG Pei-pei LIU He-xing LI Zai-feng LIU Da-qun |
| Institution: | College of Plant Protection, Hebei Agricultural University, Baoding 071001, China; Department of Biochemistry, Baoding University, Baoding 071000, China |
| Abstract: | Lr26 and Lr19 are two known important wheat leaf rust resistance genes. The positive and negative markers of the two genes have been developed at present. A total of 17 commercial wheat cultivars collected from Sichuan province were inoculated with 14 Chinese Puccinia triticina races for gene postulation in the greenhouse and used to detect Lr26 and Lr19 by multiplex PCR, respectively. The results showed that Lr26 was detected in six wheat cultivars, and no cultivar was found to carry the Lr19 gene. The multiplex PCR can be widely used for marker-assisted selection of Lr26 and Lr19. |
| Keywords: | wheat leaf rust resistance gene molecular marker multiplex PCR |
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