| 膜分离技术在鱼腥草芩蓝口服液制备工艺中的应用 |
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| 引用本文： | 陆安,杜红娜,刘炳炜,郭宽,李芳,郭永红,郭永国.膜分离技术在鱼腥草芩蓝口服液制备工艺中的应用[J].中国兽药杂志,2018,52(10):50-56. |
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| 作者姓名： | 陆安 杜红娜 刘炳炜 郭宽 李芳 郭永红 郭永国 |
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| 作者单位： | 河北维尔利动物药业集团有限公司;河北省功能性饲料添加剂工程技术研究中心;石家庄市兽用功能性添加剂工程技术研究中心 |
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| 基金项目： | 石家庄市重大科技专项研发项目(编号：186500067A);石家庄市工程技术研究中心研发项目(编号：188500227A) |
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| 摘 要： | 为了考察膜分离技术对中药鱼腥草芩蓝水提液除杂效果的影响,以鱼腥草芩蓝水提液为研究对象,采用微滤、超滤和纳滤相结合的方式,对鱼腥草芩蓝水提液进行膜过滤除杂及膜浓缩工艺进行研究,并与传统水提醇沉工艺进行比较。结果显示,鱼腥草芩蓝水提液膜过滤除杂初滤、超滤和膜浓缩工艺的最佳孔径分别为:200 nm、50 kd和1.5 kd;膜过滤除杂所制备鱼腥草芩蓝口服液中绿原酸、黄芩苷平均含量分别为1.11、8.53 mg/m L,醇沉除杂工艺所制备鱼腥草芩蓝口服液中绿原酸、黄芩苷平均含量分别为0.95、5.34 mg/m L,且不同批次含量测定的RSD值均3%(n=3);膜过滤除杂工艺制备的样品进行蛋白质检测时未出现浑浊,醇沉除杂工艺制备的样品有浑浊出现;不同工艺制备的鱼腥草芩蓝口服液,稳定性均良好。综上所述,膜分离技术除杂所制备鱼腥草芩蓝口服液工艺稳定可靠、简便易行,且绿色、安全,显著降低生产成本,本文对工业化生产和推广具有指导意义。
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| 关 键 词： | 鱼腥草芩蓝口服液 膜分离技术 绿原酸 黄芩苷 蛋白质 稳定性 |
| 收稿时间： | 2018/7/4 0:00:00 |
| 修稿时间： | 2018/9/27 0:00:00 |
Application of Membrane Separation Technology in Impurity Removal Process of Yuxingcaoqinlan oralSliquids |
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| Institution: | Hebei Weierli Animal Pharmaceutical Group Co., Ltd.,,,,,, |
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| Abstract: | Taking the Yuxingcaoqinlan water extract as research object to investigate the effect of different pore diameter membranes on the impurity removing effect. Comparing with the traditional water extraction and alcohol precipitation method, research on the membrane filtration and concentration process by way of microfiltration, ultrafiltration and nanofiltration. The result indicated that the optimal purification technology of Yuxingcaoqinlan water extract was as follow: membrane aperture for microfiltration and ultrafiltration was 200 nm and 50 kd respectively, the optimal membrane aperture for membrane concentration was 1.5 kd. The contents of chlorogenic acid and baicalin of Yuxingcaoqinlan oralSliquid prepared by membrane filtration and impurity removal process were 1.11 mg/mL and 8.53 mg/mL respectively, the contents of these ingredients described above preparated by alcohol precipitation method were 0.95 mg/mL and 5.34 mg/mL respectively, and the RSD of measured content from above two methods in different batches is less than 3% (n=3). The samples prepared by membrane filtration and impurity removal process showed no turbidity when the protein was detected, and the samples prepared by alcohol precipitation method showed turbidity and the Yuxingcaoqinlan oralSliquid prepared by different processes had good stability equally. Indicating that membrane filtration technology for the impurity removal in the preparation of Yuxingcaoqinlan oralSliquid is stable, reliable, simple, green and safe, and significantly reduce the production cost, which has a guiding significance for industrial production and promotion. |
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| Keywords: | Yuxingcaoqinlan oralSliquid Membrane Separation technology Chlorogenic acid Baicalin Protein Stability |
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