| 拮抗内生芽孢杆菌BEB17分离鉴定及其挥发性物质抑菌活性分析 |
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| 引用本文： | 陈奕鹏,杨扬,桑建伟,蔡吉苗,徐春华,黄贵修.拮抗内生芽孢杆菌BEB17分离鉴定及其挥发性物质抑菌活性分析[J].植物病理学报,2018,48(4):537-546. |
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| 作者姓名： | 陈奕鹏 杨扬 桑建伟 蔡吉苗 徐春华 黄贵修 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海口 571101 |
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| 基金项目： | 国家重点研发计划子课题(2017YFD0202104);中央级公益性科研院所基本科研业务费专项(NO.2016hzs1J020);农业部现代农业人才支撑计划项目(0316001) |
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| 摘 要： | 项目组从健康香蕉植株根部中分离得到一株对多种植物病原真菌具有抑菌活性的内生细菌。结合形态特征观察、生理生化分析及分子鉴定,鉴定该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),命名为BEB17。双皿对峙实验结果表明,该菌株产生的挥发性有机物(Volatile organic compounds,VOCs)可抑制多种病原真菌生长,其中对香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense race 4,Foc4)抑制效果最强,抑制率达66.86%±0.90%,并能抑制其孢子萌发。用BEB17产生的挥发性物质做熏蒸处理后,Foc4菌丝发生缢缩膨大,细胞膜被破坏。采用顶空法固相微萃取结合气质联用(HS-SPMEGC-M S)对BEB17产生的挥发性有机物(VOCs)进行收集鉴定,得到10类33种单体有机物组分。
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| 关 键 词： | 内生细菌 鉴定 挥发性物质 抑菌活性分析 |
Isolation and identification of antagonistic endophytic bacillus BEB17 and analysis of antibacterial activity of volatile organic compounds |
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| CHEN Yi-peng,YANG Yang,SANG Jian-wei,CAI Ji-miao,XU Chun-hua,HUANG Gui-xiu.Isolation and identification of antagonistic endophytic bacillus BEB17 and analysis of antibacterial activity of volatile organic compounds[J].Acta Phytopathologica Sinica,2018,48(4):537-546. |
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| Authors: | CHEN Yi-peng YANG Yang SANG Jian-wei CAI Ji-miao XU Chun-hua HUANG Gui-xiu |
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| Institution: | Environment and Plant Protection Institute, CATAS/Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, China |
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| Abstract: | An endogenous bacterium with antibacterial activity against various pathogenic fungi was isolated from the roots of healthy banana plants. This strain was identified as Bacillus amyloliquefaciens by morphological observation, physiological and biochemical analysis and molecular identification, and named BEB17. The experi-mental results of two inverse face-to-face petri dishes showed that the volatile organic compounds (VOCs) produced by this strain can inhibit the growth of various pathogenic fungi, which exhibited the strongest inhibitory effect on banana fusarium wilt (Fusarium oxysporum f. sp. cubense race 4, Foc4) and the inhibition rate was 66.86%±0.90%. Meanwhile, it can inhibit the spore germination of F. oxysporum. Upon VOCs fumigation treatment, the mycelium of Foc4 became constricted and dilated, and the cell membrane was also damaged. In this article, 10 kinds of 33 compounds from the VOCs produced by BEB17 were identified through headspace solid phase microextraction and GC-MS. |
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| Keywords: | endogenous bacteria identification volatile organic compounds antimicrobial activity analysis |
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