| 对番茄枯萎病具防效的海洋源赤杆菌YH-07的发酵条件探究 |
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| 引用本文： | 唐彤彤,孙星,董元华,刘勤.对番茄枯萎病具防效的海洋源赤杆菌YH-07的发酵条件探究[J].中国生物防治学报,2018,34(5):753-761. |
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| 作者姓名： | 唐彤彤 孙星 董元华 刘勤 |
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| 作者单位： | 1. 中国科学院南京土壤研究所, 南京 210008;2. 中国科学院大学, 北京 100049;3. 滁州学院, 滁州 239000 |
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| 基金项目： | 国家重点研发计划（2017YFD0200604）；国家自然科学基金（31400464） |
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| 摘 要： | 一株分离自连云港海域的赤杆菌Erythrobacter sp.YH-07对番茄枯萎病病原菌有较好的平板抑菌效果。通过单因子水平试验、正交试验对该菌株的培养基配方及摇瓶发酵条件进行优化，最优培养基配方为酵母浸膏0.25%、蔗糖1%、蛋白胨1%、NaCl 3%和MgSO4·7H2O 0.03%；最优发酵条件组合为培养温度30℃，140 r/min摇床培养，接种量1%，装液量50 mL/250 mL，初始pH 7.0。优化后的平板抑菌能力提高了9.4%。温室盆栽试验表明，赤杆菌YH-07对番茄枯萎病有显著抑制作用，防治效果保持在50%以上。同时显著降低番茄根际土的真菌数量，增加细菌和放线菌数量。综上所述，赤杆菌YH-07能有效防治番茄枯萎病害，改善根际微生物数量结构，具有进一步开发应用的前景。
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| 关 键 词： | 赤杆菌 番茄枯萎病 发酵条件优化 拮抗作用 生防效果 |
| 收稿时间： | 2018-02-01 |
Optimization of Fermentation Conditions for Erythrobacter sp. YH-07 Suppressing Fusarium Wilt of Tomato |
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| TANG Tongtong,SUN Xing,DONG Yuanhua,LIU Qin.Optimization of Fermentation Conditions for Erythrobacter sp. YH-07 Suppressing Fusarium Wilt of Tomato[J].Chinese Journal of Biological Control,2018,34(5):753-761. |
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| Authors: | TANG Tongtong SUN Xing DONG Yuanhua LIU Qin |
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| Institution: | 1. Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Chuzhou University, Chuzhou 239000, China |
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| Abstract: | A marine bacteria Erythrobacter sp. YH-07 with high biocontrol activity against Fusarium oxysporum was isolated from Lianyungang beach. We used single factor experiments and orthogonal experiments to investigate the best condition for fermentation of strain YH-07. The best composition of the medium was 0.25% (W/V) yeast extract, 1% (W/V) source, 1% (W/V) tryptone, 3% (W/V) NaCl and 0.03% (W/V) MgSO4·7H2O. The optimal fermentation conditions were the combination of temperature 30℃, initial pH7.0, 140 r/min, 1% inoculums volume percentage and medium volume 50 mL/250 mL. Under the optimum fermentation medium and culture conditions, the inhibitory rate of strain YH-07 was 9.4% higher than before. Pot experiments indicated that H7+R treatment could significantly lower the incidence rate of Fusarium wilt disease up to 50%, increase the total number of bacteria and actinomycetes, and decrease the number of fungi in rhizosphere soil. Strain YH-07 can effectively control Fusarium wilt of tomato and improve microbial community structure in rhizosphere, and it has potential development and application prospect. |
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| Keywords: | Erythrobacter sp tomato Fusarium wilt optimization of fermentation condition antagonism biocontrol efficiency |
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