| 猪脑心肌炎病毒GXLC株的分离及其3D基因分子特征的分析 |
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| 引用本文： | 陈进喜,施开创,黄胜斌,陆文俊,屈素洁,郑敏,李军.猪脑心肌炎病毒GXLC株的分离及其3D基因分子特征的分析[J].中国畜牧兽医,2011,38(1). |
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| 作者姓名： | 陈进喜 施开创 黄胜斌 陆文俊 屈素洁 郑敏 李军 |
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| 作者单位： | 1. 广西钦州市动物疫病预防控制中心,广西钦州,535000
2. 广西动物疫病预防控制中心,广西南宁,530001 |
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| 基金项目： | 广西科学基金项目(桂科青0728047); 广西科技创新能力与条件建设基金项目(08-05-01D) |
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| 摘 要： | 采集临床疑似脑心肌炎死亡仔猪的组织作为接种材料,接种于BHK-21细胞系,观察细胞病变(CPE),并用RT-PCR和间接荧光抗体试验(IFA)进行鉴定,证实分离到1株脑心肌炎病毒(encephalomyocarditis virus,EMCV),命名为GXLC株。应用RT-PCR方法扩增GXLC株的3D基因,扩增产物克隆入pMD18-T载体后进行测序,对获得的3D基因序列进行分析。序列分析结果表明,GXLC株3D基因全长1380 nt,编码460个氨基酸,含有7个抗原表位。同源性分析结果表明,GXLC株与国内外其它EMCV分离株3D基因核苷酸序列的同源性在84.7%~99.7%之间,氨基酸序列的同源性在96.1%~99.6%之间。遗传进化分析结果表明,基于3D基因核苷酸序列绘制的系统进化树可将所有EMCV分离株分成2个群:Ⅰ群和Ⅱ群,Ⅰ群可再细分为Ⅰa亚群和Ⅰb亚群,其中猪源EMCV在Ⅰ群和Ⅱ群中均有分布,而鼠源EMCV分布在Ⅰ群,人源EMCV分布在Ⅱ群;GXLC株与其它中国分离株均属于Ⅰa亚群。
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| 关 键 词： | 猪 脑心肌炎病毒 分离 3D基因 分子特征 |
Isolation of Porcine Encephalomyocarditis Virus GXLC Strain and Analysis of the Molecular Characteristics of its 3D Gene |
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| CHEN Jin-xi,SHI Kai-chuang,HUANG Sheng-bin,LU Wen-jun,QU Su-jie,ZHENG Min,LI Jun.Isolation of Porcine Encephalomyocarditis Virus GXLC Strain and Analysis of the Molecular Characteristics of its 3D Gene[J].China Animal Husbandry & Veterinary Medicine,2011,38(1). |
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| Authors: | CHEN Jin-xi SHI Kai-chuang HUANG Sheng-bin LU Wen-jun QU Su-jie ZHENG Min LI Jun |
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| Institution: | CHEN Jin-xi1,SHI Kai-chuang2,HUANG Sheng-bin2,LU Wen-jun2,QU Su-jie2,ZHENG Min2,LI Jun2(1.Qinzhou Center for Animal Disease Control and Prevention,Qinzhou 535000,China,2.Guangxi Center for Animal Disease Control and Prevention,Nanning 530001,China) |
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| Abstract: | The clinical tissue samples collected from piglets died of suspected encephalomyocarditis were homogenated and the supernatant was inoculated into baby hamster kidney(BHK-21) cell lines to isolate encephalomyocarditis virus(EMCV).According to the cytopathic effect(CPE) and the results of RT-PCR and indirect fluorescent antibody test(IFA),one strain of EMCV was successfully isolated and designated as GXLC strain.The 3D gene of GXLC strain was amplified by RT-PCR,cloned into pMD18-T vector,sequenced and analy... |
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| Keywords: | swine encephalomyocarditis virus isolation 3D gene molecular characteristic |
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