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| 广西片形吸虫中间宿主-小土窝螺线粒体rrnL基因遗传多态性研究 | |
| 引用本文： | 王树艳,李鑫,李娟,沈前程,朱兴全,黄维义.广西片形吸虫中间宿主-小土窝螺线粒体rrnL基因遗传多态性研究[J].中国畜牧兽医,2011,38(1). |
| 作者姓名： | 王树艳 李鑫 李娟 沈前程 朱兴全 黄维义 |
| 作者单位： | 1. 广西大学动物科技学院,广西南宁,530005;华南农业大学兽医学院,广东广州,510642 2. 华南农业大学兽医学院,广东广州,510642 3. 广西大学动物科技学院,广西南宁,530005 4. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州,730046 |
| 基金项目： | 教育部“长江学者和创新团队发展计划”创新团队项目(IRT0723) |
| 摘 要： | 为研究广西小土窝螺不同地理株线粒体大亚基(large subunit ribosomal rRNA,rrnL)基因的遗传多态性,对广西大片吸虫6个流行地区96个小土窝螺样品的rrnL基因进行PCR扩增,通过单链构象多态性(single strand conformation poly-morphism,SSCP)技术筛选出具有代表性的样品进行克隆、测序,并用DNAStar 5.0及MEGA 4.0软件加以比对分析。结果显示,6个地区小土窝螺rrnL基因PCR扩增长度约500 bp,在长度为273 bp的序列中检测到15个变异位点,占核苷酸总数的5.49%。百色、南宁和桂林3个地区相同地理株间存在较大的遗传差异。种系发育分析表明,线粒体rrnL基因在一定程度上不是研究小土窝螺种群遗传变异的良好分子标记。本研究为阐明片形吸虫中间宿主—小土窝螺的种群遗传关系提供了基础数据。 |
| 关 键 词： | 小土窝螺(Galba pervia) 线粒体rrnL基因 PCR-SSCP 序列分析 |
DNA Polymorphism in the Partial Mitochondrial rrnL Gene among Galba pervia(the Intermediate Host of Fasiola)Isolates from Guangxi |
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| WANG Shu-yan,LI Xin,LI Juan,SHEN Qian-cheng,ZHU Xing-quan,HUANG Wei-yi.DNA Polymorphism in the Partial Mitochondrial rrnL Gene among Galba pervia(the Intermediate Host of Fasiola)Isolates from Guangxi[J].China Animal Husbandry & Veterinary Medicine,2011,38(1). | |
| Authors: | WANG Shu-yan LI Xin LI Juan SHEN Qian-cheng ZHU Xing-quan HUANG Wei-yi |
| Institution: | WANG Shu-yan1,2,LI Xin2,LI Juan2,SHEN Qian-cheng1,ZHU Xing-quan3,HUANG Wei-yi1(1.College of Animal Science and Technology,Guangxi Univesity,Nanning 530005,China,2.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,3.Lanzhou Veterinary Research Institute/State Key Laboratory of Veterinary Etiological Biology,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
| Abstract: | In order to analyze sequence variation in the partial mitochondrial large subunit ribosomal rRNA gene(rrnL) of Galba pervia from different geographical locations in Guangxi,rrnL genes were amplified from G.pervia isolates obtained from 6 endemic origins.After screening by SSCP technique,representative samples with variable banding patterns were selected for sequencing.DNAStar 5.0 and MEGA 4.0 were used to analyze the sequences subsequently.The results showed that a fragment of the rrnL approximately 500 bp ... |
| Keywords: | Galba pervia mitochondrial rrnL gene PCR-SSCP sequence analysis |
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