| 拟南芥2-甲基-6-叶绿基-1,4-苯醌甲基转移酶启动子在转基因烟草中的表达特性分析 |
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| 引用本文： | 刘 宾,王 磊,张 伟.拟南芥2-甲基-6-叶绿基-1,4-苯醌甲基转移酶启动子在转基因烟草中的表达特性分析[J].中国农学通报,2007,23(4):33-33. |
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| 作者姓名： | 刘 宾 王 磊 张 伟 |
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| 作者单位： | 1. 河北大学生命科学学院,河北,保定,071002;中国农业科学院生物技术研究所,北京,100081
2. 中国农业科学院生物技术研究所,北京,100081 |
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| 基金项目： | 国家重点基础研究发展规划（973项目）资助“大豆应用核心种质构建与基因多样性”（2004CB17203）. |
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| 摘 要： | 通过对报告基因GUS产物的分析进行了拟南芥2-甲基-6-叶绿基-1,4-苯醌甲基转移酶（MPBQ MT）启动子在转基因烟草中的表达特性的初步研究。构建含该启动子和GUS报告基因的植物表达载体，通过农杆菌介导转化烟草，对转基因烟草进行GUS组织化学染色和GUS荧光定量分析该启动子表达特性。GUS在转基因烟草的根和种子中基本不表达，茎上有一定表达，叶上表达量最高，约是茎的4.7~10.9倍。结果表明MPBQ MT基因启动子主要在烟草绿色组织中特异性高表达。
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| 关 键 词： | 生态农业 生态农业 六位一体 模式 系统效益 |
| 修稿时间： | 2007-02-022007-02-08 |
Characterization of Arabidopsis thaliana 2-methyl-6-phytyl-1,4-benzoquinol Methyltransferase (MPBQ MT) Gene Promoter in Transgenic Tobacoo |
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| Liu Bin,Wang Lei,Zhang Wei.Characterization of Arabidopsis thaliana 2-methyl-6-phytyl-1,4-benzoquinol Methyltransferase (MPBQ MT) Gene Promoter in Transgenic Tobacoo[J].Chinese Agricultural Science Bulletin,2007,23(4):33-33. |
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| Authors: | Liu Bin Wang Lei Zhang Wei |
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| Institution: | 1.College of Life Sciences, He bei University, Baoding 071002; 2.Biotechnolgy Research Institute, Chinese Academy of Agricultural Science, Beijing 100081 |
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| Abstract: | The expression pattern of the promoter of Arabidopsis gene 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT) in transgenic tobacco was studied according to the analysis of GUS reporter gene. The promoter was fused with GUS reporter gene and this expression cassette was introduced into wild tobacco by Agrobacterium-mediated transformation. The expression pattern of the promoter was analyzed by GUS staining and quantitative fluorometric GUS assay. The GUS staining wasn't observed in roots and seeds of transgenic tobacco, which was visualized in stems and leaves. The GUS expression was the highest in leaves, which was about 4.7~10.9 times of stems. MPBQ MT promoter was expressed preferentially in the green tissues of tobacco. |
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| Keywords: | Tobacco Vitamin E 2-methyl-6-phytyl-1 4-benzoquinol methyltransferase (MPBQ MT) Promoter GUS-staining |
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