| 莆田近海孔烂病海带微生物群落结构及其与环境因子的关系 |
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| 引用本文： | 冯磊,钟晨辉,林琪,唐隆晨,宦忠艳.莆田近海孔烂病海带微生物群落结构及其与环境因子的关系[J].水产学报,2022,46(9):1721-1731. |
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| 作者姓名： | 冯磊 钟晨辉 林琪 唐隆晨 宦忠艳 |
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| 作者单位： | 福建省水产研究所，上海海洋大学,福建省水产研究所,福建省水产研究所,福建省水产研究所,福建省水产研究所 |
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| 基金项目： | 现代农业产业技术体系建设专项(CARS-50)；福建省科技重大专项(2019NZ08003)；福建省水产种业创新与产业化工程项目(2017FJSCZY01) |
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| 摘 要： | 为探究莆田近海孔烂病海带微生物群落结构及其与环境因子的关系，本研究采用16S rRNA扩增子测序技术对健康海带(HT)、孔烂病海带(DT)、健康海带养殖区海水(HS)和孔烂病海带养殖区海水(DS)样本进行测序分析，利用冗余分析研究了海水微生物群落与环境因子的关系。结果显示，HT和DT样本的丰富度指数(ace)、香农指数(shannon)和均匀度指数(heip)均具有显著性差异，HS和DS样本的丰富度指数和香农指数有显著性差异，均匀度指数则无显著性差异，各样本的微生物群落分区明显。微生物群落组成分析显示，HT和DT样本群落组成的差异主要由盐单胞菌科产生，而HS和DS样本群落组成的差异主要由蓝细菌门和假交替单胞菌科决定。环境因子关联分析显示，盐度和温度与微生物群落的关联在HS和DS样本中具有显著性差异，对海水微生物群落分布影响较大。海水微生物中的优势菌蓝细菌与盐度，与温度呈负相关；优势菌科黄杆菌科、红杆菌科、假交替单胞菌科和弧菌科与盐度呈负相关，与温度呈正相关。研究表明，孔烂病的发生可能与海带微生物中的盐单胞菌和海水微生物中的蓝细菌和假交替单胞菌有密切的联系，盐度和温度可能是导致海水微生物群落发生变化的关键环境因子。
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| 关 键 词： | 海带 孔烂病 高通量测序 群落结构 环境因子 |
| 收稿时间： | 2021/1/30 0:00:00 |
| 修稿时间： | 2021/3/2 0:00:00 |
Microbial community structure and its relationship with environmental factors on Hole-Rotten disease of Saccharina japonica in coastal Putian |
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| FENG Lei,ZHONG Chenhui,LIN Qi,TANG Longchen,HUAN Zhongyan.Microbial community structure and its relationship with environmental factors on Hole-Rotten disease of Saccharina japonica in coastal Putian[J].Journal of Fisheries of China,2022,46(9):1721-1731. |
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| Authors: | FENG Lei ZHONG Chenhui LIN Qi TANG Longchen HUAN Zhongyan |
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| Institution: | Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361000, China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | In order to explore microbial community structure and the correlation between environmental factors and community structure on Hole-Rotten disease of kelp Saccharina japonica in coastal regions of Putian, in this study, 16S rRNA gene amplicon sequencing technology was used to analyze the samples of healthy (HT) S. japonica, S. japonica with Hole-Rotten disease (DT), seawater from area of cultivated healthy S. japonica (HS) and seawater from area of cultivated S. japonica with Hole-Rotten disease (DS). The interactions between seawater microbial and environmental factors were analyzed by redundancy analysis (RDA) as well. The results showed that the Ace index, Shannon index and Heip index were significantly different between HT and DT samples (P<0.05), the Ace index and Shannon indes were significantly different between HS and DS samples (P<0.05), while the Heip index was not significantly different (P>0.05). The results of principal co-ordinate analysis showed that the microbial community of each sample was obviously partitioned. Microbial community composition analysis showed the relative abundance of Halomonadaceae in the HT and DT samples were 1.63% and 49.01%, the relative abundance of Cyanobacteria in the HS and DS samples were 74.45% and 3.89%, respectively. The relative abundance of Pseudoalteromonadaceae in DS samples is 30.87%, nevertheless, less than 1% in HS samples. The results of correlation analysis of environmental factors showed the salinity and temperature were significantly different in the association between HS and DS samples (P<0.05), and the explanations for the variation of seawater microbial communities were 46.75% and 42.42%, respectively. The dominant Cyanobacteria in seawater was positively correlated with salinity and negatively correlated with temperature; the dominant Flavobacteriaceae, Rhodobacteraceae, Pseudoalteromonadaceae and Vibrionaceae, were negatively correlated with salinity and positively correlated with temperature. In summary, the occurrence of Hole-Rotten disease may be intimately related to the Halomonas in S. japonica epiphytes and the Cyanobacteria and Pseudoalteromonadaceae in seawater microorganisms. Salinity and temperature might be the key environmental factors leading to the change of seawater microflora. |
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| Keywords: | Saccharina japonica Hole-Rotten disease hight-throughput sequencing community structure environmental factors |
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