|
|||||
|
|
| 牛病毒性腹泻病毒抗原捕获ELISA检测方法的标准化研究 | |
| 引用本文： | 邓宇,王新华,郭燕,钟发刚,沈敏.牛病毒性腹泻病毒抗原捕获ELISA检测方法的标准化研究[J].中国预防兽医学报,2007,29(7):541-544,549. |
| 作者姓名： | 邓宇 王新华 郭燕 钟发刚 沈敏 |
| 作者单位： | 1. 西昌学院,动物科学系,四川,西昌,615013;新疆农垦科学院,新疆,石河子,832000 2. 新疆农垦科学院,新疆,石河子,832000 3. 新疆农垦科学院,新疆,石河子,832000;石河子大学,动物科技学院,新疆,石河子,832003 |
| 基金项目： | 科技部农业科技成果转化基金 |
| 摘 要： | 用纯化牛病毒性腹泻病毒免疫蛋鸡制备出的卵黄抗体作为包被抗体,采用自制的单抗为一抗,建立牛病毒性腹泻病毒抗原捕获ELISA方法。通过试验确定,抗牛病毒性腹泻病毒卵黄抗体最佳包被浓度为1:50;McAb最适稀释浓度为1:10,HRP-羊抗鼠IgG工作浓度为1:800。通过引入牛病毒性腹泻病毒质控血清进行质控检验,该方法所得检测结果均在质量控制范围内,达到预定标准化要求。标准化的抗原捕获ELISA方法具有特异、灵敏、可靠、方便、快捷等特点,可广泛应用推广,为我国牛病毒性腹泻病毒监测提供了行之有效的技术手段。 |
| 关 键 词： | 牛病毒性腹泻病毒 抗原捕获ELISA 标准化 |
| 文章编号： | 1008-0589（2007）07-0541-04 |
| 修稿时间： | 2006-11-15 |
Development of Ag-capture ELISA for detecting bovine viral diarrhea virus |
|
| DENG Yu,WANG Xin-hua,GUO Yan,ZHONG Fa-gang,SHEN Min.Development of Ag-capture ELISA for detecting bovine viral diarrhea virus[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(7):541-544,549. | |
| Authors: | DENG Yu WANG Xin-hua GUO Yan ZHONG Fa-gang SHEN Min |
| Institution: | 1, Department of Animal Science, Xichang College, Xichang 615013, China; 2. Xinjiang Academy of Agriculture and Reclamation Science, Shihezi 832000, China; 3. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China |
| Abstract: | An Ag-capture ELISA was developed using anti-BVDV IgY as coating antibody and self-made McAb as the first antibody.The optimal concentration of anti-BVDV IgY for coating was at 1:50 dilution,with an optimal McAb dilution of 1:10 and HRP-goat anti mouse IgG dilution of 1:800.This assay was standardized against reference serum for quality controls and the P/N value was consistent with the standards of analogic method.The results showed that the Ag-capture ELISA kit has excellent stability.It is highly specific,sensitive and reproducible for detecting bovine viral diarrhea virus(BVDV)antigen.Therefore this method could be widely applied for BVDV detection. |
| Keywords: | bovine viral diarrhea virus Ag-capture ELISA standardization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |