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| Antibiotic Resistance and Heavy Metals Tolerance in Gram-Negative Bacteria from Diseased American Bullfrog （Rana catesbeiana） Cultured in Malaysia | |
| 作者姓名： | M Najiah S W Lee W Wendy L W Tee M Nadirah S H Faizah |
| 作者单位： | M Na-jian(Department of Fisheries Science and Aquaculture, Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, Kuala;Terengganu 21030, Malaysia;Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, Kuala Terengganu 21030, Malays);S W Lee(Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Pengkalan Chepa 16100, Malaysia);W Wendy,L W Tee,M Nadirah(Department of Fisheries Science and Aquaculture, Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, Kuala;Terengganu 21030, Malaysia);S H Faizah(Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, Kuala Terengganu 21030, Malaysia) |
| 基金项目： | Acknowledgements This project was funded by Universiti Malaysia Terengganu, Malaysia. |
| 摘 要： | A total of 140 bacterial isolates have been successfully isolated from various organs of diseased American bullfrog （Rana catesbeiana） cultured in Malaysia. The most frequently isolated bacteria was Edwardsiella spp. （46 isolates） followed by Aeromonas spp. （33 isolates）, Flavobacterium spp. （31 isolates）, and Vibrio spp. （30 isolates）. Majority of the bacterial isolates were found sensitive to furazolidone （85.0%）, chloramphenicol （85.0%）, oxolinic acid （90.0%）, florfenicol （95.0%）, and flumequine （97.5%）. On the other hand, most of the bacterial isolates were resistant to oleandomycin （77.5%） and lincomycin （87.5%）. Nitrofurantoin and flumequine can be inhibited the growth of all of Vibrio spp. whereas all isolates of Edwardsiella spp. were found sensitive to florfenicol and flumequine. Multiple antibiotic resistance （MAR） index were in range of 0.30-0.40, indicating that bacterial isolates from cultured bullfrogs may have received high risk exposure to the tested antibiotics. In addition, 90-100% of the isolates were resistant to copper, cadmium, and chromium. These results provided insight information on tolerance level of bacterial isolates from cultured bullfrogs to 21 antibiotics as well as heavy metals. |
| 关 键 词： | 牛蛙 患病 细菌 金属 |
| 收稿时间： | 15 December 2008 |
Antibiotic Resistance and Heavy Metals Tolerance in Gram-Negative Bacteria from Diseased American Bullfrog (Rana catesbeiana) Cultured in Malaysia |
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| M Najiah,S W Lee,W Wendy,L W Tee,M Nadirah,S H Faizah.Antibiotic Resistance and Heavy Metals Tolerance in Gram-Negative Bacteria from Diseased American Bullfrog (Rana catesbeiana) Cultured in Malaysia[J].Agricultural Sciences in China,2009,8(10):1270-1275. | |
| Authors: | M Na-jian S W Lee W Wendy L W Tee M Nadirah S H Faizah |
| Institution: | 1. Department of Fisheries Science and Aquaculture, Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, Kuala;Terengganu 21030, Malaysia;Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, Kuala Terengganu 21030, Malays 2. Faculty of Agro Industry and Natural Resources, Universiti Malaysia Kelantan, Pengkalan Chepa 16100, Malaysia 3. Department of Fisheries Science and Aquaculture, Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, Kuala;Terengganu 21030, Malaysia 4. Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, Kuala Terengganu 21030, Malaysia |
| Abstract: | A total of 140 bacterial isolates have been successfully isolated from various organs of diseased American bullfrog (Rana catesbeiana) cultured in Malaysia. The most frequently isolated bacteria was Edwardsiella spp. (46 isolates) followed by Aeromonas spp. (33 isolates), Flavobacterium spp. (31 isolates), and Vibrio spp. (30 isolates). Majority of the bacterial isolates were found sensitive to furazolidone (85.0%), chloramphenicol (85.0%), oxolinic acid (90.0%), florfenicol (95.0%), and flumequine (97.5%). On the other hand, most of the bacterial isolates were resistant to oleandomycin (77.5%) and lincomycin (87.5%). Nitrofurantoin and flumequine can be inhibited the growth of all of Vibrio spp. whereas all isolates of Edwardsiella spp. were found sensitive to florfenicol and flumequine. Multiple antibiotic resistance (MAR) index were in range of 0.30-0.40, indicating that bacterial isolates from cultured bullfrogs may have received high risk exposure to the tested antibiotics. In addition, 90-100% of the isolates were resistant to copper, cadmium, and chromium. These results provided insight information on tolerance level of bacterial isolates from cultured bullfrogs to 21 antibiotics as well as heavy metals. |
| Keywords: | Rana catesbeiana MAR index Edwardsiella Aeromonas Flavobacterium Vibrio antibiotic resistance heavy metal tolerance |
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