Random amplified polymorphic DNA analysis of Australian rice (Oryza sativa L.) varieties |
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| Authors: | H L Ko D C Cowan R J Henry G C Graham A B Blakeney L G Lewin |
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| Institution: | (1) Queensland Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, The University of Queensland, 4072, QLD, Australia;(2) New South Wales Department of Agriculture, Yanco Agricultural Institute, 2073 Yanco, NSW, Australia |
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| Abstract: | Summary The genetic relationships between rice varieties were analysed by using the polymerase chain reaction (PCR), with arbitrary oligonucleotide primers in the random amplified polymorphic DNA (RAPD) method. PCR with 22 arbitrary primers applied to 37 varieties produced 144 useful markers, of which 67% were polymorphic. Thus, with selected primers sufficient polymorphism could be detected to allow identification of individual varieties. Visual examination of electrophoresis gels and analysis of banding patterns confirmed that commercial Australian and USA lines and their relatives were very closely related, with similarity indices of 88–97%. Three varieties originating from more distant geographical centres were easily distinguished, producing variety-specific amplification profiles and expressing a lower similarity index of 80% to all other varieties tested. PCR offers a potentially simple, rapid and reliable method for rice genotype identification and recognition of lines that could contribute genetic diversity to new commercial varieties.Abbreviations PCR
Polymerase Chain Reaction
- RAPD
Random Amplified Polymorphic DNA |
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| Keywords: | genetic diversity polymerase chain reaction rice random amplified polymorphic DNA Oryza sativa variation |
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