| 一个新的水稻温敏感叶色突变体基因定位分析 |
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| 引用本文： | 祁鲁,刘晓,陈佳颖,林冬枝,董彦君,徐建龙.一个新的水稻温敏感叶色突变体基因定位分析[J].核农学报,2010,24(2):220-224. |
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| 作者姓名： | 祁鲁 刘晓 陈佳颖 林冬枝 董彦君 徐建龙 |
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| 作者单位： | 1. 上海师范大学生命与环境科学学院植物遗传与功能基因研究室,上海,200234
2. 中国农业科学院作物科学研究所,北京,100081 |
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| 基金项目： | 国家航天育种工程项目，上海市科委项目 |
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| 摘 要： | 粳稻品种嘉花1号种子经60Coγ射线辐照后,筛选得到一个温敏感叶色突变体MR20。该突变体的幼苗叶色在较低温度(20℃)下呈黄白色,并随着温度升高叶色由黄白逐渐转绿,其临界温度约为22.5℃,在低温(20℃)和高温(32℃)条件下叶绿素含量也存在显著差异,表明该突变体为低温导致叶绿素合成受阻的温敏感叶色突变体。遗传分析表明该突变体性状受一对隐性核基因控制,暂命名为tsc(t)。以该突变体与籼稻9311杂交的F2群体作为定位群体,利用SSR分子标记将tsc(t)基因初步定位在水稻第3染色体短臂上的分子标记RM231和RM14407之间,其遗传距离分别为1.5cM和0.5cM。随后,进一步扩大F2群体将该tsc(t)基因定位在分子标记MM0541和RM14407之间的约440kb内。
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| 关 键 词： | 水稻 基因定位 温敏感 叶绿素缺失突变体 |
| 收稿时间： | 2009-08-06 |
MAPPING OF A NEW THERMO-SENSITIVE LEAF-COLOR MUTANT GENE IN RICE (Oryza sativa L.) |
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| QI Lu,LIU Xiao,CHEN Jia-ying,LIN Dong-zhi,DONG Yan-jun,XU Jian-long.MAPPING OF A NEW THERMO-SENSITIVE LEAF-COLOR MUTANT GENE IN RICE (Oryza sativa L.)[J].Acta Agriculturae Nucleatae Sinica,2010,24(2):220-224. |
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| Authors: | QI Lu LIU Xiao CHEN Jia-ying LIN Dong-zhi DONG Yan-jun XU Jian-long |
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| Institution: | 1.Laboratory of Plant Genetics and Functional Genes, College of Life and Environment Sciences, Shanghai Normal University, Shanghai200234;
2.The Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing100081 |
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| Abstract: | A thermo-sensitive leaf-color mutant,MR20,was discovered in progeny of a japonica variety,Jiahua No.1,induced by 60Co γ-rays irradiation.Under the conditions of low temperature (20℃),the leaf-color of the mutant at seedling stage was yellow and tuned into green with the increase of temperature and its critical temperature was about 22.5℃.In addition,the significant difference of the chlorophyll contents at two temperatures (20℃ and 32℃) was observed showing that MR20 was a thermo-sensitive chlorophyll-deficit mutant at the seedling stage.Genetic analysis revealed that the character of thermo-sensitive chlorophyll-deficit was controlled by a single recessive nuclear gene,tentatively designated as tsc(t).The F2 population derived from the cross MR20×9311 was as materials,and SSR markers was used,the tsc(t) gene was roughly mapped at between RM231 and RM14407 molecular markers on the short arm of chromosome 3 with the genetic distance of 1.5cM and 0.5cM,respectively.With larger F2 mapping population,the tsc(t) gene was narrowed to 440kb between MM0541 and RM14407 molecular markers. |
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| Keywords: | rice gene mapping:thermo-sensitive chlorophyll-deficit mutant |
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