| 辽东湾斑海豹线粒体苏氨酸和脯氨酸及控制区部分序列分析 |
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| 引用本文： | 韩家波,赫崇波,王效敏,王强,马志强,周遵春,王丕烈.辽东湾斑海豹线粒体苏氨酸和脯氨酸及控制区部分序列分析[J].水产科学,2007,26(2):74-78. |
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| 作者姓名： | 韩家波 赫崇波 王效敏 王强 马志强 周遵春 王丕烈 |
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| 作者单位： | 1. 辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁省应用海洋生物技术开放实验室,辽宁,大连,116023
2. 辽宁师范大学,实验分析中心,辽宁,大连,116029 |
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| 基金项目： | 国家"908"项目辽宁专项 |
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| 摘 要： | 通过PCR产物测序法,测定了46只辽东湾斑海豹线粒体DNA的一段包括部分苏氨酸和脯氨酸tRNA基因及部分控制区的717 bp序列,得到17个序列单元型。与港海豹相比,所有辽东湾斑海豹17个单元型在控制区序列中都有2处缺失,是区分港海豹和斑海豹的种间分子标记。与日本海和鄂霍次克海区斑海豹m tDNA相同片段进行比较研究,发现辽东湾斑海豹的线粒体控制区DNA的单元型比例相对较少,遗传多样性水平相对较低。辽东湾斑海豹m tDNA的16296位点即苏氨酸tRNA基因序列的最后一位有1个C碱基的插入,16607位点存在T/C转换,这两个变异位点是鉴别辽东湾斑海豹与日本及鄂霍次克海区斑海豹的重要分子标记。初步表明,辽东湾斑海豹与日本海、鄂霍次克海斑海豹是属于不同的地理种群,或者辽东湾斑海豹同这两个海区斑海豹未发生基因交流或者雌性个体的相互交流。
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| 关 键 词： | 辽东湾 斑海豹 线粒体 种群遗传 |
| 文章编号： | 1003-1111(2007)02-0074-05 |
| 修稿时间： | 2006-02-21 |
Sequence Analysis of Mitochondrial tRNAThr, tRNAPro and Control Region from Spotted Seals (Phoca largha) in Liaodong Gulf |
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| HAN Jia-bo,HE Chong-bo,WANG Xiao-min,WANG Qiang,MA Zhi-qiang,ZHOU Zun-chun,WANG Pi-lie.Sequence Analysis of Mitochondrial tRNAThr, tRNAPro and Control Region from Spotted Seals (Phoca largha) in Liaodong Gulf[J].Fisheries Science,2007,26(2):74-78. |
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| Authors: | HAN Jia-bo HE Chong-bo WANG Xiao-min WANG Qiang MA Zhi-qiang ZHOU Zun-chun WANG Pi-lie |
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| Institution: | 1. Liaoning Ocean and Fisheries Science Research Institute, Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Open Lab of Applied Marine Biotechnology, Dalian 116023,China; 2. Experiment Analysis Center, Liaoning Normal University, Dalian 116029, China |
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| Abstract: | The sequences of mtDNA control region,adjacent proline and a portion of the threonine tRNA genes were sequenced in 46 spotted seals using PCR-based sequencing method.The length was 717 bp,and 17 haplotypes were obtained from the sequences.Compared with its closely related species,all the 17 haplotype sequences from spotted seals had two deletions in mtDNA control region,which could be used as a species-specific marker between spotted seals and harbor seals.Compared with spotted seals along Sea of Japan and Sea of Okhotsk,spotted seals in Liaodong Gulf have relative less haplotypes and lower genetic diversity,and have one insertion at site 16296 of mtDNA,which could be a marker between these populations.The results showed that the spotted seals in Liaodong Gulf maybe belong to an independent population and no gene flow or exchange of female individuals occurred between those along Sea of Japan and Sea of Okhotsk. |
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| Keywords: | Liaodong Gulf Phoca largha mtDNA population genetics |
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