| 番茄抗黄化曲叶病基因在不同温度下对TYLCV复制的影响 |
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| 引用本文： | 李佳蔚,林铭,胡鸿,王孝宣,国艳梅,黄泽军,杜永臣,高建昌.番茄抗黄化曲叶病基因在不同温度下对TYLCV复制的影响[J].园艺学报,2016,43(1):71-79. |
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| 作者姓名： | 李佳蔚 林铭 胡鸿 王孝宣 国艳梅 黄泽军 杜永臣 高建昌 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，北京 100081 |
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| 基金项目： | 国家自然科学基金，国家‘863’计划项目，农业部园艺作物生物学与种质创制重点实验室项目 |
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| 摘 要： | 采用番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)侵染克隆接种技术和实时荧光定量PCR方法,从TYLCV在番茄叶片内复制繁殖的角度,系统研究了不同环境温度下单个和多个抗性基因叠加对病毒复制的影响,以期为合理进行抗病基因整合,选育抗TYLCV的番茄新品种提供理论指导。结果显示,(1)春季温室栽培环境下,含Ty-1/Ty-3的番茄材料能抑制病毒复制,接种后28 d其体内病毒含量仅是感病材料病毒含量的千分之一;秋季温室栽培环境下,这种抑制作用降低,病毒含量与感病材料相当。精确控温种植的含Ty-1/Ty-3的近等基因系番茄材料中病毒的含量变化趋势与此相同。(2)含Ty-2的番茄材料在春秋两季栽培环境下,均表现出对病毒复制的抑制作用,接种后28 d其体内病毒含量仅为感病材料病毒含量的万分之一。(3)同时含有2个基因(Ty-1和Ty-2)和多个基因(Ty-1、Ty-2和Ty-3)的番茄材料在抑制病毒复制方面不存在累加效应。
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| 关 键 词： | 番茄 番茄黄化曲叶病毒 荧光定量PCR |
The Combined Effects of Different TYLCV Resistant Genes and Temperatures on TYLCV Duplication |
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| LI Jia-wei,LIN Ming,HU Hong,WANG Xiao-xuan,GUO Yan-mei,HUANG Ze-jun,DU Yong-chen,GAO Jian-chang.The Combined Effects of Different TYLCV Resistant Genes and Temperatures on TYLCV Duplication[J].Acta Horticulturae Sinica,2016,43(1):71-79. |
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| Authors: | LI Jia-wei LIN Ming HU Hong WANG Xiao-xuan GUO Yan-mei HUANG Ze-jun DU Yong-chen GAO Jian-chang |
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| Institution: | Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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| Abstract: | Tomato yellow leaf curl disease（TYLCD）has already been a major constraint to tomato production in China. It is caused by Tomato yellow leaf curl virus（TYLCV）and several other begomoviruses. These begomoviruses replicate their genome quickly in tomato plants after infection and transport to whole plant to result in disease. Development of TYLCV resistance variety is economic and efficient way to control TYLCD. To provide theoretical bases to the breeding for TYLCV resistance，we detected the combined effects of different TYLCV resistance genes and temperatures on TYLCV replicate TYLCV in tomato lines with Ty-1/ Ty-3 was one thousandth of that in susceptible tomato lines. However，after infiltration of infectious clone TYLCV-IL CN：SH2] using real-time PCR. TYLCV resistance genesTy-1/ Ty-3 acted inhibitory effect on TYLCV replication in solar greenhouse in spring. The amount of TYLCV in tomato lines with Ty-1/ Ty-3 was one thousandth of that in susceptible tomato lines. However the inhibition of Ty-1/ Ty-3 on TYLCV replication was decreased in solar greenhouse in autumn. The copy number of TYLCV in tomato materials with Ty-1/ Ty-3 and susceptible tomato materials showed a similar trend of variation. Similarly，under temperature treatments，Ty-1/ Ty-3 inhibited sharply the replication of TYLCV at low temperature，but not at high temperature. In contrast to Ty-1/ Ty-3，the inhibition of Ty-2 on the replication of TYLCV was more severe and stable. The amounts of TYLCV in tomato lines with Ty-2 were one ten-thousandth of that in susceptible tomato lines in solar greenhouse both in spring and in autumn. Furthermore，there were not addictive effect between the two genes（Ty-1 and Ty-2）or among the three genes（Ty-1，Ty-2 and Ty-3）. |
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| Keywords: | tomato TYLCV real-time PCR |
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