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| 尼古丁降解菌L1的分离鉴定与降解特性分析 | |
| 引用本文： | 雷丽萍,夏振远,王玥,魏海雷,刘杏忠.尼古丁降解菌L1的分离鉴定与降解特性分析[J].农业生物技术学报,2007,15(4):721-722. |
| 作者姓名： | 雷丽萍 夏振远 王玥 魏海雷 刘杏忠 |
| 作者单位： | 1. 云南省烟草科学研究所,玉溪,653100 2. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100101;北京林业大学生物科学与技术学院,北京,100083 3. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100101 |
| 基金项目： | 国家自然科学基金;云南省烟草专卖局资助项目 |
| 摘 要： | 通过对分离于烟草叶片的154株具有降解尼古丁细菌菌株筛选，获得能高效降解尼古丁的L1菌株。通过形态观察，16S rDNA序列分析和生理生化测定将其鉴定为Bacillus simplex。菌落生长密度测定和高压液相色谱分析表明， L1菌株最适生长尼古丁浓度为1g/L，随着菌落密度增加，尼古丁降解效率逐渐升高，36 h最高降解率达到75.0%。而低尼古丁含量不利于L1菌株的生长，过高尼古丁含量对L1菌株的生长和降解效率具有反馈抑制作用。菌株L1降解尼古丁过程中无色素产生，说明其尼古丁代谢途径有别于节杆菌。 |
| 关 键 词： | 尼古丁 芽孢杆菌 生物降解 |
| 文章编号： | 1006-1304（2007）04-0721-02 |
| 收稿时间： | 2006-09-25 |
| 修稿时间： | 2006-10-16 |
Isolation and Characterization of Nicotine-degrading Bacterial Strain L1 |
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| LEI Li-ping,XIA Zhen-yuan,WANG Yue,WEI Hai-lei,LIU Xing-zhong.Isolation and Characterization of Nicotine-degrading Bacterial Strain L1[J].Journal of Agricultural Biotechnology,2007,15(4):721-722. | |
| Authors: | LEI Li-ping XIA Zhen-yuan WANG Yue WEI Hai-lei LIU Xing-zhong |
| Institution: | 1. Yunnan Tobacco Science Research Institute, Yuxi 653100, China; 2. Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; 3. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China |
| Abstract: | One handred fifty-four bacteria were isolated from tobacco leaves on the medium with nicotine as unique carbon source. A high nicotine-degrading bacterium (L1) was obtained after screening. Based on morphology, physiological and chemical tests, 16S rDNA sequence and phylogenetic analysis, L1 was identified as Bacillus simplex. The concentration of nicotine for the optimal growth of L1 was 1.0g/L. The nicotine was degraded up to 75% by strain L1 under the optimized incubation conditions for 36 h monitored by high-performance liquid chromatography. There was no pigment observed at degrading process. This study demonstrates that Bacillus simplex L1 had strong ability to degrade nicotine, and the degrading mechanism may be different from Arthrobacter sp. |
| Keywords: | nicotine Bacillus simplex biodegradation |
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