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| 五味子根、藤茎及叶提取物中五味子甲素、乙素的含量测定 | |
| 引用本文： | 魏忠宝,孙成贺,侯微,王英平,胡晶晶,姜艳玲.五味子根、藤茎及叶提取物中五味子甲素、乙素的含量测定[J].特产研究,2009,31(4):56-58. |
| 作者姓名： | 魏忠宝 孙成贺 侯微 王英平 胡晶晶 姜艳玲 |
| 作者单位： | 1. 长春中医药大学,吉林,长春,130017 2. 中国农业科学院特产研究所,吉林,吉林,132109 3. 中国药科大学,江苏,南京,210009 |
| 基金项目： | 科技部支撑计划，吉林省科技厅项目 |
| 摘 要： | 目的采用HPLC法测定五味子根、藤茎及叶不同溶剂提取物中五味子甲素、乙素的含量,同时筛选出最佳提取溶剂,为扩大五味子的入药部位资源提供理论依据。方法采用Hypersil ODS2（4.6mm×250mm,5μm）色谱柱;流动相：A.甲醇、B.水,梯度洗脱,0～20min,70%～80%A、20～35min,80%～70%A;流速：1mL/min;检测波长：254nm。结果五味子根中五味子甲素、乙素的含量最高,乙醇为最佳提取溶剂。 |
| 关 键 词： | HPLC 五味子 五味子根 五味子藤茎 五味子叶 |
Determination of Deoxyschizandrin and Schisandrin in Extracts from the Schisandra chinensis Baill Root, Rattan and Leaves |
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| WEI Zhong-bao,SUN Cheng-he,HOU Wei,WANG Ying-ping,HU Jing-jing,JIANG Yan-ling.Determination of Deoxyschizandrin and Schisandrin in Extracts from the Schisandra chinensis Baill Root, Rattan and Leaves[J].Special Wild Economic Animal and Plant Research,2009,31(4):56-58. | |
| Authors: | WEI Zhong-bao SUN Cheng-he HOU Wei WANG Ying-ping HU Jing-jing JIANG Yan-ling |
| Institution: | WEI Zhong-bao,SUN Cheng-he,HOU Wei,WANG Ying-ping,HU Jing-jing,JIANG Yan-ling(1.Changchun University of Traditional Chinese Medicine,Changchun 130017,China;2.Instituteof Special Wild Economic Animal and Plant Science,CAAS.,Jilin 132109,China;3.China Pharmaceutical University,Nanjing 210009,China) |
| Abstract: | Objective To establish a HPLC method for the determination of deoxyschizandrin and schisandrin levels in extracts from the Schisandra chinensis roots,rattans and leaves using different solvents.Select the best extraction solvent.Provide a theoretical basis for widening resources of Schisandra chinensis.Method Determination was performed on a Hypersil ODS2 column（4.6 mm×250 mm,5 μm）.The mobile phase：methanol-water gradient elution with the flowrate of1 mLl/min.The wavelength was set at 254 nm.Results Determination of deoxyschizandrin and schisandrin levels in extracts from the Schisandra chinensis roots is the highest.Ethanol is the best extraction solvent. |
| Keywords: | HPLC |
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