| 筛选产纤维素酶菌株及其产酶条件的优化 |
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| 引用本文： | 葛江丽,施汉钰,刘瑰琦,刘芳,曹昊.筛选产纤维素酶菌株及其产酶条件的优化[J].安徽农业科学,2014(30):10441-10442,10455. |
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| 作者姓名： | 葛江丽 施汉钰 刘瑰琦 刘芳 曹昊 |
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| 作者单位： | 黑龙江省牡丹江林业科学研究所,黑龙江牡丹江,157009 |
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| 摘 要： | 目的]筛选降解菌糠纤维素的菌株,并且优化其发酵条件.方法]采用纤维素-刚果红培养基进行筛选,筛选出纤维素酶活较高的菌株.通过比较不同氮源、碳氮比例等条件下CMC酶活,研究其最佳发酵条件.结果]选出一株酶活较高的菌株,命名为N3.其最适有机氮源为豆饼粉,无机氮源是（NH4）2SO4.最适合N3产酶的（NH4）2SO4∶豆饼粉比例为2∶4.最适碳源氮源比例为5∶2.结论]N3是一株具有研究价值的产纤维素酶的菌株.
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| 关 键 词： | 纤维素酶 筛选 发酵优化 |
Screening of Cellulose Producer Strains and Optimization of the Conditions for Cellulose Production |
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| Institution: | GE Jiang-li, SHI Han-yu, LIU Gui-qi et al (Mudajiang Forestry Research Institute, Mudanjiang, Heilongjiang 157009) |
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| Abstract: | Objective ] To screen strains producing cellulose and optimize the culture conditions. Method ] With Cellulose Gango Red Medium in screening, a high-yield cellulose strain was obtained. The culture conditions were optimized by comparing the CMCase on the kinds and ration of nitrogen source. Result] N3 was isolated. The highest level of cellulose activity was induced in the medium containing organic nitrogenous （ soybean cake） and inorganic nitrogen （ ammonium sulfate） with the ration of 4： 2. The ratio of C to N is 5： 2. Conclusion ] N3 had a highly research value. |
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| Keywords: | Cellulose Screening Fertilization and optimization |
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