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| 鸡卵泡颗粒细胞转染Bcl-2基因对IGF-1分泌的影响 | |
| 引用本文： | 周东蕊,杨利国,刘君华.鸡卵泡颗粒细胞转染Bcl-2基因对IGF-1分泌的影响[J].南京农业大学学报,2005,28(3):133-136. |
| 作者姓名： | 周东蕊 杨利国 刘君华 |
| 作者单位： | 1. 南京农业大学动物科技学院,江苏,南京,210095 2. 华中农业大学动物科技学院,湖北,武汉,430070 3. 山东省安丘市畜牧局,山东,安丘,262100 |
| 基金项目： | 国家自然科学基金资助项目（30070555） |
| 摘 要： | 选用正在产蛋的罗曼鸡，取体积排序第一（F1）和第四（F4）的卵泡，分离颗粒细胞层细胞。采用脂质体介导方法将来源于F1和F4卵泡的颗粒细胞用PBS（对照）、空载体或Bcl-2重组质粒处理，然后在不完全无血清培养基（不添加胰岛素）中培养48和96h，用流式细胞术检测Bcl-2蛋白表达，用放射免疫测定技术检测IGF-1的含量。结果发现，转染Bcl-2重组质粒组的Bcl-2蛋白表达量和IGF-1分泌量均多于其他各组。分析来源于不同卵泡期卵泡颗粒细胞的分泌功能，发现随时间的增加F1细胞IGF-1分泌量减少，而F4的IGF-1分泌量基本不变。结果表明，在鸡卵泡颗粒细胞中转染的Bcl-2基因具有表达功能，表达的蛋白具有调节鸡卵泡颗粒细胞分泌IGF-1的作用；鸡卵泡颗粒细胞IGF-1的分泌还受鸡卵泡的大小和颗粒细胞在体外培养时间的影响。 |
| 关 键 词： | 鸡 Bcl-2基因 IGF-1 卵泡颗粒细胞 转染 |
| 文章编号： | 1000-2030(2005)03-0133-04 |
| 收稿时间： | 06 2 2004 12:00AM |
| 修稿时间： | 2004年6月2日 |
Effect of Bcl-2 gene on IGF-1 in vitro secretion in follicular granulosa cell of laying hens |
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| ZHOU Dong-rui,YANG Li-guo,LIU Jun-hua.Effect of Bcl-2 gene on IGF-1 in vitro secretion in follicular granulosa cell of laying hens[J].Journal of Nanjing Agricultural University,2005,28(3):133-136. | |
| Authors: | ZHOU Dong-rui YANG Li-guo LIU Jun-hua |
| Abstract: | Granulosa cells isolated from follicles ranked the first(F_1)and fourth(F_4)in size of each laying Romen hen were treated(or transfected)with PBS(control),vector transfection and Bcl-2 recombined plasmid in combination of liposome respectivey.Expression of Bcl-2 gene and content detection of IGF-1(insulin like growth factor-1)were detected by flow cytometry and radioimmunoassay respectively when the cells were incubated with non-serum medium without insulin for 48 h and 96 h post-transfection.The results indicated that the gene treatment group had a greater expression of Bcl-2 and content of IGF-1 than the other two groups.Analyzing the secretion function of granulosa cells original from F_1 and F_4 follicles we found that IGF-1 content in F_1 cells decreased and F_4 cells kept constant as incubation time.These results showed that gene expression was exhibited in chicken follicular granulosa cell transfected by Bcl-2 gene,indicating Bcl-2 gene played a role in regulating IGF-1 secretion in follicular granulosa of chicken in vitro.At the same time,IGF-1 secretion was regulated by the size of follicles and the culture time in vitro. |
| Keywords: | hen Bcl-2 gene IGF-1 follicular granulosa cell transfection |
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