| 鸭病毒性肝炎病毒的分离鉴定及其病原特性研究 |
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| 引用本文： | 高亚东,凌红丽,董瑞娥,孙海新.鸭病毒性肝炎病毒的分离鉴定及其病原特性研究[J].安徽农业科学,2010,38(34):19506-19508. |
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| 作者姓名： | 高亚东 凌红丽 董瑞娥 孙海新 |
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| 作者单位： | 青岛康地恩药业宝依特生物制药有限公司,山东青岛266114 |
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| 基金项目： | 青岛市城阳区科技发展计划项目 |
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| 摘 要： | 目的]调查鸭病毒性肝炎的病原,并分析近年来该病不断发生和难以控制的原因。方法]从山东临沂、潍坊、滨州等地区鸭场有鸭肝炎典型症状的雏鸭的肝、脾中分离病毒,通过鸡胚和鸭胚接种、RT-PCR检测、血清学试验、攻毒试验等研究其病原特性。结果]分离到4株鸭病毒性肝炎病毒（DHV）,第5代鸭胚分离毒的ELD50为103.41～105.20,与传统的Ⅰ型DHV的血清交叉保护率为20%～80%。分离株对4日龄雏鸭的致死率为50%～100%。攻毒后雏鸭均出现典型的鸭肝炎症状,24～48 h出现死亡高峰。结论]DHV分离株的毒力存在地区差异。
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| 关 键 词： | 鸭病毒性肝炎病毒 分离 鉴定 毒力 |
Studies on the Isolation, Identification and Pathogenic Characteristic of Duck Hepatitis Virus |
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| Institution: | GAO Ya-dong et al(Qingdao Contient Pharmaceutical Boitech Biological Pharmaceutical Co.,Ltd.,Qingdao,Shandong 266114) |
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| Abstract: | Objective] To determine the pathogen of duck viral hepatitis which is prevalent recently and to analyze the reasons for difficult control.Method] Viruses were isolated from livers and spleens of ducks in farms of Linyi,Weifang and Binzhou of Shandong Province which displayed typical clinical symptoms of duck viral hepatitis.Their pathogenic characteristics were studied by chicken or duck embryo inoculation,RT-PCR,serological test,and duck regression.Result] Four duck hepatitis virus(DHV) strains were isolated,and the 5th passage allantoic fluid contained 103.41-105.20 ELD50 /ml.They had serum cross protection rates of 20%-80% against DHV type I.The mortality of 4-day-old healthy ducks challenged by these four stains was 50%-100%.All challenged ducks had typical lesions of duck viral hepatitis,and a peak appeared after 24-48 h post challenge.Conclusion] The virulence of DHV isolates has regional difference. |
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| Keywords: | Duck hepatitis virus Identification Isolation Virulence |
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