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仔猪腹泻大肠埃希菌的分离鉴定及药敏试验
引用本文:仔猪腹泻大肠埃希菌的分离鉴定及药敏试验.仔猪腹泻大肠埃希菌的分离鉴定及药敏试验[J].畜牧与饲料科学,2014,35(3):127-127.
作者姓名:仔猪腹泻大肠埃希菌的分离鉴定及药敏试验
作者单位:[1]甘肃省武威市凉州区动物疫病预防控制中心,甘肃凉州733000 [2]甘肃省武威市凉州区高坝镇畜牧站,甘肃凉州733000
摘    要:为了了解甘肃省武威市凉州区部分地区引起仔猪黄白痢的致病性大肠埃希菌的主要血清型及其致病性,从凉州区4家规模化猪场采集了60份仔猪肛门拭子,用细菌分离培养方法分离出20株大肠埃希菌菌株,然后经形态观察、生化试验,确定了该次分离菌株为致病性大肠埃希菌。经血清型鉴定,表明20株分离菌株分布在8个血清型中,其中优势血清型为O8(9株),占总数的45%。药物敏感性试验结果表明,分离菌株对临床常用药物具有抗药性。该研究能够对凉州区仔猪腹泻病的流行病学研究、诊断和免疫防制提供科学参考。


Separation,Identification and Drug Sensitivity Test of Escherichia coli in Diarrhea Piglets
Abstract:In order to understand main serotypes and their pathogenicity of pathogenic Escherichia coli that caused yellow-white dysentery of piglets in Liangzhou Prefecture, Wuwei City of Gansu Province, 60 anal swabs of piglets were collected from 4 large-scale pig farms in Liangzhou Prefecture. And 20 strains of E. coli were separated by the bacterial isolation and culture methods. And the separated strains were identified as pathogenic E. coli by morphological observation and biochemical test. The results of serotype identification showed that 20 isolated strains were distributed in eight serotypes. And the dominant serotype was O8 (9 strains), which took 45%of total isolated strains. The results of drug sensitivity test showed that the isolated strains had resistance to common clinical drugs. The research could provide scientific references for the epidemiologic study, diagnosis and immunological control of piglet diarrhea in Liangzhou Prefecture.
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