转Xa21基因圣稻301的培育及其田间表现 |
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引用本文: | 姜明松,李广贤,宫德英,姚方印.转Xa21基因圣稻301的培育及其田间表现[J].山东农业科学,2003(6):10-11,14. |
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作者姓名: | 姜明松 李广贤 宫德英 姚方印 |
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作者单位: | 山东省水稻研究所,山东,济宁,272017 |
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摘 要: | 利用农杆菌介导的遗传转化系统 ,将白叶枯病抗性基因Xa2 1导入山东主栽品种圣稻 30 1胚性愈伤组织 ,从T0 至T5代 ,利用GUS染色和分子标记辅助逐代选择并进行田间抗病虫性鉴定 ,获得了大量纯合稳定的转基因圣稻 30 1,并对其田间抗性及农艺性状表现进行了调查研究
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关 键 词: | 转基因水稻 圣稻301 Xa21基因 田间表现 |
文章编号: | 1001-4942(2003)06-0010-02 |
Breeding and field performance of Xa21 transgenic japonica variety Shengdao301 |
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Abstract: | Using a genetic transformation system mediated by Agrobacterium tumefaciens, Xa21 gene against the bacterial blight disease was introduced into calli of a japonica variety Shengdao301 cultivated in Shandong Province. Based on Gus detection, marker assisted selection and inoculation from T_0 to T_5 generation, a great number of homozygous transgenic Shengdao301 plants were obtained. Performance of field resistance and agronomy traits were investigated. |
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Keywords: | Transgenic japonica Shengdao301 Xa21 gene field performance |
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