| GC/MS法研究猪体内盐酸克伦特罗的残留代谢规律 |
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| 引用本文： | 鲍伟华.GC/MS法研究猪体内盐酸克伦特罗的残留代谢规律[J].中国兽药杂志,2009,43(11):26-29. |
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| 作者姓名： | 鲍伟华 |
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| 作者单位： | 吕燕,杨挺,赵健(宁波市农业科学研究院,浙江宁波,315040);鲍伟华,余晓华,孙泽祥,徐震宇(宁波市畜牧兽医局,浙江宁波,315012);姜柏芳,孙志康(余姚市畜牧兽医局,浙江余姚,315400) |
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| 基金项目： | 宁波市农业科技攻关项目 |
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| 摘 要： | 样品用乙酸乙酯提取,盐酸溶液萃取后,再经SCX固相萃取柱净化,50℃水浴中氮气吹干后衍生化。以气相色谱-质谱选择86、212、262、277四个碎片离子进行定性定量分析。盐酸克伦特罗在0.01～2.0 mg/L成线性（R2为0.999）,猪肉中3种不同添加浓度0.01、0.10和0.20mg/kg下克仑特罗平均回收率分别为88.31%、92.85%、93.97%,相对标准偏差分别为5.26%、7.58%、4.59%,样品中最低检测限（S/N=3）为0.1 ug/kg。应用该方法研究了克仑特罗在猪体内的消除并初步探讨其代谢机理。结果表明,在饲喂浓度（3 mg/kg）1个月后,猪体内盐酸克伦特罗蓄积量不算太高,最高蓄积浓度为493.59 ug/kg,而且停药后代谢又非常快。
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| 关 键 词： | 猪 盐酸克伦特罗 残留 代谢 气相色谱-质谱法(GC/MS) |
| 收稿时间： | 2009/5/27 0:00:00 |
| 修稿时间： | 2009/7/31 0:00:00 |
Studies on the Residue Elimination of clenbuterol in pig Using GC/MS |
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| baoweihua.Studies on the Residue Elimination of clenbuterol in pig Using GC/MS[J].Chinese Journal of Veterinary Drug,2009,43(11):26-29. |
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| Authors: | baoweihua |
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| Institution: | Director of Ningbo Animal Hushandry veterinary Service |
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| Abstract: | The clenbuterole was extracted from samples by employing ethyl acetate and hydrochloric acid solution, and the cleaning procedure was performed by SCX solid phase extraction cartridge. The extract was warmed with water at 50 ℃ and blowed with nitrogen until it got near to dryness. Then it was derivated. Retention time and specificions were used to identify melamine. The selective fragments were m/z 86, 212, 262, 277 respectively. The calibration curve of clenbuterol was linear （ correlation coefficient ： 0. 999 ） within the range of 0.01 - 2.0 mg/L. The average recoveries at 0.01,0.10 and 0.20 mg/kg were 88.31% , 92.85% , 93.97%. The relative standard deviations （RSD） of the method was lower than 8% for each case, and the limit of detection （LOD）, defined in terms of 3 times baseline nosie was 0.1 ug/kg respectively. The developing method was applied to study the metabolize behavior and mechanism of clenbuterol in pig. The results indicated that the residual of melamine was found in pig while starting to feed, the content of clenbuterol in pig was increased gradually, and the highest content of clenbuterol was 493.59 ug/kg, but the content of clenbuterol in pig was reduced gradually immediately while stoping feed. |
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| Keywords: | pig clenbuterol residual elimination gas chromatography-mass spectrometry（GC/MS） |
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