|
|||||
|
|
| 胸腺肽αl原核表达载体的构建及在大肠杆菌中的表达 | |
| 引用本文： | 崔雪志,马凤龙,乔彦良,刘焕奇,任庆娜,刘焕珍.胸腺肽αl原核表达载体的构建及在大肠杆菌中的表达[J].中国农学通报,2008,24(7):22-25. |
| 作者姓名： | 崔雪志 马凤龙 乔彦良 刘焕奇 任庆娜 刘焕珍 |
| 作者单位： | 1. 青岛农业大学动物科技学院,青岛,266109;山东信得科技股份有限公司,青岛,266061 2. 山东信得科技股份有限公司,青岛,266061 3. 青岛农业大学动物科技学院,青岛,266109 |
| 摘 要： | 构建胸腺肽αl（Thymosin αl，Tαl）基因原核表达载体，并在大肠杆菌BL21中进行表达，为大量获得胸腺肽αl打下基础。将人工合成的Tαl三串体基因插入到表达载体pET32a后，CaC12法转化大肠杆菌BL21，经氨苄青霉素筛选后用IPTG诱导表达，SDS-PAGE检测BL21中胸腺肽αl的表达。大肠杆菌中检测到与目的蛋白相对分子量(31kD)相符的条带。成功构建了Tαl原核表达载体，该蛋白能在大肠杆菌中表达。 |
| 关 键 词： | 小麦 小麦 水分 品质性状 相关性 |
| 收稿时间： | 2008-05-06 |
| 修稿时间： | 2008-05-14 |
Construct Prokaryotic Expression Vector of Thymosin Alpha l and Expression in Escherichia coli |
|
| Cui Xuezhi,Ma Fenglong,Qiao Yanliang,Liu Huanqi,Ren Qingn,Liu Huanzhen.Construct Prokaryotic Expression Vector of Thymosin Alpha l and Expression in Escherichia coli[J].Chinese Agricultural Science Bulletin,2008,24(7):22-25. | |
| Authors: | Cui Xuezhi Ma Fenglong Qiao Yanliang Liu Huanqi Ren Qingn Liu Huanzhen |
| Institution: | Department of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109; Sinder Medicine Qingdao, Qingdao 266061 |
| Abstract: | To construct prokaryotic expression vector of thymosin alpha l (Tαl), and express in Escherichia coli strain BL21, make foundation to gain amounts of thymosin alpha l (Tαl). The four repeats of thymosin alpha l (Tαl), which was chemically synthesized, was inserted into expression vector pET32a. The recombined pET32a-3Tαl was transferred into Escherichia coli strain BL21with CaCl2. After screening with Ampicillin, the positive clone was induced to express with IPTG, and the expression product was analyzed with SDS-PAGE. A positive protein band, which was the same as interest protein molecular weight about 31kD, was found in the expression product. The gene of Tαl was cloned into the expression vector correctly and was expressed successfully in E. coli expression system. |
| Keywords: | thymosin alpha lzz expression vector pET32azz SDS-PAGEzz |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国农学通报》浏览原始摘要信息 | |
| 点击此处可从《中国农学通报》下载免费的PDF全文 | |