| 苎麻4-香豆酸辅酶A连接酶-1基因的克隆与分析 |
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| 引用本文： | 陈建荣,郭清泉.苎麻4-香豆酸辅酶A连接酶-1基因的克隆与分析[J].中国农学通报,2008,24(10):83-87. |
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| 作者姓名： | 陈建荣 郭清泉 |
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| 作者单位： | 长沙学院生物工程与环境科学系,长沙,410003 |
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| 摘 要： | 以苎麻为材料，采用PCR方法，以简并引物从苎麻基因组DNA模板中，首次克隆了4-香豆酸辅酶A连接酶-1基因（4CL-1）的DNA部分序列，长度为983 bp；采用RT-PCR的方法，以特异引物，从苎麻茎RNA反转录的cDNA为模板中，首次克隆了苎麻4CL-1cDNA核心序列，长度为110 bp；经生物信息学方法分析，得知所获得的基因序列编码一段含37个氨基酸残基的多肽，该多肽与几个酰基合成酶和AMP结合酶的同源性较高，可以推论其编码了AMP形成与结合的保守区域。
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| 关 键 词： | 大豆 大豆 超高产 潜力 展望 |
| 收稿时间： | 2008-08-15 |
| 修稿时间： | 2008-08-20 |
Cloning and Analysis of the 4-coumarate-CoA Lignase Gene-1 |
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| CHEN Jian-Rong.Cloning and Analysis of the 4-coumarate-CoA Lignase Gene-1[J].Chinese Agricultural Science Bulletin,2008,24(10):83-87. |
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| Authors: | CHEN Jian-Rong |
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| Abstract: | Boehmeria nivea (Linn.) Gaud. cultivar “Zhongzhu No1” has been used, 4CL-1 genomic DNA sequence has been cloned first time by PCR with degenerate primers, the length of the sequence is 983 bp; 4CL-1 cDNA sequence has been cloned first time by RT-PCR with special primers from ramie stem, the length of the sequence is 110 bp; Bioinformatics analysis shows that this sequence encode a polypeptide about 37 amino acid residue, this polypeptide is similar to acyl-CoA synthetases, and related to AMP forming and binding. |
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| Keywords: | Boehmeria nivea (Linn ) Gaudzz lignin biosynthesiszz 4-coumarate-CoA ligase(4CL)zz gene cloningzz |
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