| 孟加拉红和水溶性色浆脱色真菌的筛选及其脱色工艺优化(英文) |
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| 引用本文： | 魏宝阳,沈炜,田云,高国赋,卢向阳,曾秀军.孟加拉红和水溶性色浆脱色真菌的筛选及其脱色工艺优化(英文)[J].农业科学与技术,2010(8):164-168. |
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| 作者姓名： | 魏宝阳 沈炜 田云 高国赋 卢向阳 曾秀军 |
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| 作者单位： | [1]生物科学技术学院,湖南长沙410128 [2]湖南省农业科学院科技情报研究所,湖南长沙410125 |
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| 基金项目： | 国家”十一五”科技支撑计划（2008BADC4808）;科技部国际科技合作项目（2010DFA62510）;研究生创新基金项目（54040108003）. |
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| 摘 要： | 以从土壤中通过富集培养分离得到的2号菌株为对象,采用液体培养研究其在不同培养基、不同的碳、氮源、起始pH值、培养温度等条件下对孟加拉红和水溶性色浆的脱色效果。结果表明在以豆芽汁培养基和PDA培养基为基本培养基,蔗糖为碳源,硝酸铵为氮源,初始pH值为6.0～8.0时脱色效果最佳。根据菌株形态和ITS序列分析,初步鉴定该菌为黄曲霉。
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| 关 键 词： | ITS序列 染料 脱色 黄曲霉 |
Screening of Decolorizing Fungi for Rose Bengal and Water-soluble Colour Paste and Its Decolorization Conditions |
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| WEI Bao-yang,SHEN Wei,TIAN Yun,GAO Guo-fu,LU Xiang-yang,ZENG Xiu-jun.Screening of Decolorizing Fungi for Rose Bengal and Water-soluble Colour Paste and Its Decolorization Conditions[J].Agricultural Science ＆ Technology,2010(8):164-168. |
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| Authors: | WEI Bao-yang SHEN Wei TIAN Yun GAO Guo-fu LU Xiang-yang ZENG Xiu-jun |
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| Institution: | 1. College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128; 2. Institute of Scientific and Technological Information,Hunan Academy of Agricultural Sciences,Changsha 410125 |
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| Abstract: | The strain No. 2 which was isolated from the soil through enrichment culture was used as the experimental material. It was cultured in liquid medium to research decolorizing effect to Rose Bengal and soluble color paste under the different conditions of different media,carbon sources,nitrogen sources,initial pH values and culture temperatures. The results revealed that the optimum decolorizing conditions were using bean juice medium and PDA medium as the minimal medium,sucrose as the carbon source,and ammonium nitrate as the nitrogen source,initial pH 6.0-8.0. In addition,the strain was primarily identified as Aspergillus flavus according to its morphous and ITS sequence analysis. |
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| Keywords: | ITS sequence Dyestuff Decolorization Aspergillus flavus |
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