| SSR标记对宁单11种子纯度的鉴定 |
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| 引用本文： | 李苗,李新,甘晓燕,陈晓军,宋玉霞.SSR标记对宁单11种子纯度的鉴定[J].种子,2010,29(9). |
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| 作者姓名： | 李苗 李新 甘晓燕 陈晓军 宋玉霞 |
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| 作者单位： | 宁夏农业生物技术重点实验室;宁夏农林科学院农作物研究所; |
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| 基金项目： | 宁夏回族自治区科技攻关项目，宁夏回族自治区自然基金项目 |
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| 摘 要： | 采用SSR分子标记方法,选用24对引物,以杂交玉米宁单11杂交种子及其父母本为实验材料,对宁单11种子纯度进行鉴定。通过带型分析,筛选出在杂交种中呈现清晰且双亲互补带型的特异性引物,并依据特异性引物的筛选标记结果对抽样批次的宁单11种子样本进行纯度鉴定。结果表明:phi034、phi080、umc1638、bnlg439、bnlg1792五对SSR引物可用于玉米杂交种宁单11种子纯度鉴定。
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| 关 键 词： | SSR标记 宁单11 种子纯度 |
Study on Identifying Ningdan 11 Maize Hybird Seed Purity by SSR Markers |
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| LI Miao,LI Xin,GAN Xiao-yan,CHEN Xiao-jun,SONG Yu-xia.Study on Identifying Ningdan 11 Maize Hybird Seed Purity by SSR Markers[J].Seed,2010,29(9). |
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| Authors: | LI Miao LI Xin GAN Xiao-yan CHEN Xiao-jun SONG Yu-xia |
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| Institution: | LI Miao1,LI Xin2,GAN Xiao-yan1,CHEN Xiao-jun1,SONG Yu-xia1(1.Key Lab of Agricultural Biotechnology of Ningxia,Ningxia Province.Yingchuan Ningxia 750002,China,2.Crops Institute,Ningxia Academy of Agricultural and Forestry Sciences,Yongning Ningxia 750105,China) |
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| Abstract: | In this study,the genomic DNA extracted from F1 of Ningdan 11 and its parents lines were subjected to SSR analysis with 24 pairs of primer.By zone type analysis,screened out specific primers which zone type was clear and parents complementary.According to the analysis of specific primers markers,seed purity of Ningdan11 maize hybird were identified.The result showed that five specific primer phi034,phi080,umc1638,bnlg439,bnlg1792 were suitable for identifying seed purity of this variety. |
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| Keywords: | SSR marker Ningdan11 seed purity |
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