| 蓝莓品种 ISSR 指纹图谱构建的初步研究 |
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| 引用本文： | 郑姗,张立杰,谢丽雪,张小艳,李韬.蓝莓品种 ISSR 指纹图谱构建的初步研究[J].福建省农科院学报,2014(12):1198-1201. |
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| 作者姓名： | 郑姗 张立杰 谢丽雪 张小艳 李韬 |
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| 作者单位： | 福建省农业科学院果树研究所,福建福州350013 |
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| 基金项目： | 福建省种业创新与产业化工程项目（2014S1477-22）,福建省农业科学院导师制青年科技创新基金项目（2013DQB-9） |
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| 摘 要： | 利用 ISSR 分子标记技术分析17个蓝莓品种的遗传多样性，并构建 DNA 指纹图谱。从60个 ISSR 引物中筛选出10个稳定的多态性引物。采用 NTSYS-PC 2．0软件进行聚类分析，17个蓝莓品种的遗传相似系数为0．66～0．86，在0．69的水平可分为4个品种群。引物 UBC825、UBC862可将17个蓝莓品种区分开，并依此建立了数字化的 DNA 指纹图谱，为蓝莓品种分类、快速鉴定提供依据。
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| 关 键 词： | 蓝莓 ISSR 指纹图谱 遗传多样性 |
DNA Fingerprinting by ISSR Markers for 17 Blueberries |
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| Authors: | ZHENG Shan ZHANG Li-jie XIE Li-xue ZHANG Xiao-yan LI Tao |
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| Institution: | (Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China) |
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| Abstract: | Inter-simple sequence repeat （ISSR）analysis was applied for the determination of the genetic diversity, as well as the establishment of the DNA fingerprints,for 17 selected blueberry cultivars.Ten out of 60 ISSR primers were chosen for the study.Cluster analysis revealed that the genetic similarity coefficients of the 17 samples ranged from 0.66 to 0.86. Using the coefficient threshold of 0.69,4 classifications were formed for the blueberries. They could be positively distinguished by 2 ISSR primers （i.e.,UBC825 and UBC862 ）,and used to obtain the digital DNA fingerprints. |
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| Keywords: | blueberry ISSR fingerprint genetic diversity |
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