| 河北省南部鸡肝周炎大肠杆菌的分离及血清型鉴定 |
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| 作者姓名： | 马兴树 朱美霞 王斌 王艳春 米青荣 |
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| 作者单位： | 河北工程大学农学院分子生物学实验室 |
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| 基金项目： | 河北工程大学博士基金? |
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| 摘 要： | 通过对2003年3月～2008年4月,自河北省邯郸市、邢台市、石家庄市、衡水市、沧州市等发病鸡场呈现典型肝周炎病变的病死鸡肝脏分离的132株致病性大肠杆菌进行了生化特性测定,O抗原血清型鉴定及药敏试验。结果表明132株均符合大肠杆菌生化特征,试验用55种大肠杆菌单因子阳性血清进行O血清型鉴定,确定了103株O血清型,占分离菌株数的78.03%(103/132)。选取O1、O21、O65、O78、O93血清型共15株菌株(每一血清型选取3株不同来源的菌株)做药敏试验,证实多数菌株对丁胺卡那霉素高敏,对头孢曲松中敏,而对磺胺甲硝唑耐受。
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| 关 键 词： | 鸡致病性大肠杆菌 O血清型 药敏试验 |
Isolation, Identification and Serotyping of E.coli of Chicken Periheptosis in the Southern Area of Hebei Province |
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| Authors: | Ma Xingshu Zhu Meixi Wang Bin Wang Yanchun Mi Qingrong |
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| Abstract: | Determination of biochemical characteristics, Oantigen identification, drug sensitivity test were studied on the 132 pathogenic E.coli that isolated from the typical periheptosis of chickens in Handan city, Xingtai city, Shijiazhuang city, Hengshui city, Cangzhou city in Hebei Province between March 2003 and April 2008. The results show that 132 isolates are in correspondence with biochemical characteristics of pathogenic E. coli. We have identified 103 strains which belong to 25 O-antigen types with 55 kinds of sin- gle-factor positive serum and the rate of the isolated strains was 78.03% (103/132).The drug sensitivity test used by O1, O21, O65, O78, O93 serotype of 15 strains (each serotype selected three different sources of strain) that confirmed most strains high sensitivity to amikacin, mid-sensitivity to ceftriaxone, and sulfa- metronidazole to tolerance. |
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| Keywords: | chicken pathogenic e coli o serum types drug sensitivity test |
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