| 光皮桦组织培养技术研究 |
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| 引用本文： | 陈伟,林镇斌,郑郁善.光皮桦组织培养技术研究[J].福建林业科技,2006,33(1):67-71,101. |
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| 作者姓名： | 陈伟 林镇斌 郑郁善 |
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| 作者单位： | 1. 南京林业大学,江苏,南京,210037
2. 福建农林大学,福建,福州,350002 |
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| 摘 要： | 对光皮桦带芽茎段组织培养技术的外植体启动培养、丛生芽增殖以及试管苗生根和炼苗移栽等方面开展研究,结果表明,外植体灭菌最优流程:经75%酒精1~1.5 min处理后转入0.1%HgCl2溶液消毒处理7 min;在改良MS+6-BA5.0mg.L-1+NAA 0.01 mg.L-1的培养基上,外植体启动速度最快;在改良MS+6-BA 4.5 mg.L-1+蔗糖40 g.L-1+NAA0.01 mg.L-1的培养基上,外植体增殖系数最高可达6.9倍;在改良WH+IBA 3.0 mg.L-1+NAA 1.5 mg.L-1培养基中,继代苗的生根率最高;试管苗移栽的最佳基质为沙∶田园土=1∶1。
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| 关 键 词： | 光皮桦 组织培养 无性繁殖 |
| 文章编号： | 1002-7351（2006）01-0067-05 |
| 收稿时间： | 2005-09-12 |
| 修稿时间： | 2005-09-12 |
Study on Technology of Tissue Culture in Betula luminifera |
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| CHEN Wei,LIN Zhen-bin,ZHENG Yu-shan.Study on Technology of Tissue Culture in Betula luminifera[J].Journal of Fujian Forestry Science and Technology,2006,33(1):67-71,101. |
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| Authors: | CHEN Wei LIN Zhen-bin ZHENG Yu-shan |
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| Abstract: | This article studied the tissue culture of Betula luminifera,including inducing callus,bourgeoning buds,multiplication a cluster of buds and growing root and practicing seedling in different nutrient organic.The result showed that the best proceeding of disinfecting of the stems with bud are disinfecting 75% alcohol after 1～1.5 min,treated by 0.1% HgCl_2 liquor in seven minutes;The fast initiation speed of culture medium is mend-MS+6-BA 5.0 mg·L~(-1)+NAA 0.01 mg·L~(-1);the medium with mend-MS+6-BA 4.5 mg·L~(-1)+saccharose 40 g·L~(-1)+NAA 0.01 mg·L~(-1) is best medium which multiplication coefficient is up to 6.9;The medium of the highest root rate include mend-WH+IBA 3.0 mg·L~(-1)+NAA 1.5 mg·L~(-1);the optimize transplanting medium are the sandy and garden soil which mixed with 1∶1. |
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| Keywords: | Betula luminifera tissue culture asexual propagation |
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