| 广西罗非鱼无乳链球菌的分离鉴定、毒力基因及耐药性研究 |
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| 引用本文： | 郭长明,陈怀君,袁橙,罗福广,黄德生,贾国华,袁圣,朱善元.广西罗非鱼无乳链球菌的分离鉴定、毒力基因及耐药性研究[J].中国畜牧兽医,2021,48(7):2607-2616. |
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| 作者姓名： | 郭长明 陈怀君 袁橙 罗福广 黄德生 贾国华 袁圣 朱善元 |
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| 作者单位： | 1. 江苏农牧科技职业学院, 江苏省兽用生物制药高技术研究重点实验室, 泰州 225300;2. 广西大学动物科学技术学院, 南宁 530004;3. 柳州市渔业技术推广站, 柳州 545006;4. 合浦县水产技术推广站, 北海 536100;5. 湛江恒兴养殖技术服务有限公司, 湛江 524000 |
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| 基金项目： | 江苏高校"青蓝工程"项目（苏教师函[2021]11号）；江苏省高等学校自然科学研究面上项目（19KJB230005）；泰州市科技支撑计划（农业）项目（TN201916）；泰州市311高层次人才培养工程（[2019]21号）；江苏农牧科技职业学院科研项目（NSF20190202） |
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| 摘 要： | 本研究旨在明确中国罗非鱼主养区广西各地罗非鱼无乳链球菌（Streptococcus agalactiae）分离株的血清型分布、毒力基因携带情况和耐药情况，为罗非鱼无乳链球菌病的防控奠定基础。2018-2019年从广西柳州、钦州、南宁、北海等地患无乳链球菌病罗非鱼体内分离了47株无乳链球菌，并对各分离株的血清型、毒力基因分布和耐药情况进行检测和分析。血清型检测结果表明，47株无乳链球菌血清型高度一致，均为Ⅰa血清型。对4种毒力基因检测结果表明，47株无乳链球菌临床分离株cylE、sodA、gapC毒力基因的检出率均为100%，而scpB基因仅在人源参考菌株2603V/R中检出，在所有鱼源分离株中未检出。对11类（31种）常见抗生素的药敏试验结果表明，临床分离株对磺胺异噁唑、新霉素、庆大霉素、卡那霉素的耐药率达到90%以上，对氧氟沙星、左氧氟沙星、氨苄青霉素、阿莫西林、头孢克洛、头孢曲松、头孢哌酮、头孢拉定、土霉素、强力霉素的敏感性均为100%。多重耐药检测结果表明，5重以上耐药菌株占93.62%，其中9重以上耐药菌株为19.15%，且均分离自柳州地区。结果表明，广西地区罗非鱼源无乳链球菌血清型单一，均为Ⅰa血清型，且均携带多种毒力基因，多重耐药现象严重。
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| 关 键 词： | 无乳链球菌 罗非鱼 血清型 毒力基因 耐药性 |
| 收稿时间： | 2021-01-18 |
Isolation,Identification,Virulence Genes and Antibiotic Resistance Analysis of Streptococcus agalactiae from Tilapia in Guangxi |
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| GUO Changming,CHEN Huaijun,YUAN Cheng,LUO Fuguang,HUANG Desheng,JIA Guohua,YUAN Sheng,ZHU Shanyuan.Isolation,Identification,Virulence Genes and Antibiotic Resistance Analysis of Streptococcus agalactiae from Tilapia in Guangxi[J].China Animal Husbandry & Veterinary Medicine,2021,48(7):2607-2616. |
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| Authors: | GUO Changming CHEN Huaijun YUAN Cheng LUO Fuguang HUANG Desheng JIA Guohua YUAN Sheng ZHU Shanyuan |
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| Institution: | 1. Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;2. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;3. Liuzhou Fishery Technology Extension Station, Liuzhou 545006, China;4. Hepu Aquatic Technology Extension Station, Beihai 536100, China;5. Zhanjiang Hengxing Breeding Technology Service Co., Ltd., Zhanjiang 524000, China |
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| Abstract: | The purpose of this study was to determine the serotype distribution,virulence gene carrying and drug resistance of Streptococcus agalactiae (S.agalactiae) isolated from tilapia in Guangxi,China,and laid a foundation for the prevention and control of S.agalactiae.47 strains of S.agalactiae were isolated from tilapia farms in Liuzhou,Qinzhou,Nanning and Beihai of Guangxi in 2018-2019,the serotype,distribution of virulence gene and drug resistance were detected and analyzed.The results of serotype test showed that 47 clinical isolates of S.agalactiae strains were Ⅰa serotype.The results of virulence gene detection showed that all clinical isolates of S.agalactiae carried cylE,sodA and gapC genes.The scpB gene was only detected in human reference strain 2603V/R,but not detected in all isolates of piscine S.agalactiae.The results of drug sensitivity test showed that the drug resistance rate of all the piscine isolates to sulfamethoxazole,neomycin,gentamicin and kanamycin was more than 90%,the drug sensitivity of the 47 piscineisolates to of loxacin,levofloxacin,ampicillin,amoxicillin,cefaclor,ceftriaxone,cefoperazone,cefradine,terramycin and doxycycline was 100%.All piscine strains had multiple drug resistance,and 93.62% of the 47 strains were resistanted to more than 5 kinds of drugs.Among all the piscine strains,the isolates resistanted to 9 drugs were accounted for 19.15% and isolated from Liuzhou.The results showed that the serotype of S.agalactiae isolated from tilapia in Guangxi was Ⅰa serotype.All 47 piscine isolates of S.agalactiae carried multiple virulence genes,and the phenomenon of multiple drug resistance was serious. |
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| Keywords: | Streptococcus agalactiae tilapia serotype virulence gene antibiotic resistance |
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