In vivo administration of acepromazine or promethazine to horse decreases the reactive oxygen species production response of subsequently isolated neutrophils to stimulation with phorbol myristate acetate |
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| Authors: | F PÉTERS T FRANCK M PEQUITO G de la REBIÈRE S GRULKE A SALCCICIA D VERWILGHEN L CHIAVACCINI G DEBY-DUPONT & D SERTEYN |
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| Institution: | University of Liège, Faculty of Veterinary Medicine, Equine Clinic, B41, 4000 Liège 1;;University of Liège, Institute of Chemistry, Centre for Oxygen Research &Development (C.O.R.D), B6a, 4000 Liège 1, Belgium |
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| Abstract: | The previous experiments have shown that some phenothiazines have antioxidant and anti-inflammatory properties in vitro . In this study the inhibition of the production of reactive oxygen species (ROS) by neutrophils was studied in two groups of horses, which received a dose of 0.1 mg/kg of either acepromazine or promethazine intravenously. Blood samples were collected before (T0) and 0.5, 1, 3 and 5 h after drug administration. The chemiluminescence (CML) response of neutrophils was measured ex vivo in the presence of luminol for a period of 10 min and the maximum CML value (peak value) recorded. There was a significant inhibition of the ROS production in the acepromazine treated group (49% inhibition) at 5 h after administration and in the promethazine group (24% inhibition) at 3 h after administration ( P < 0.05 vs. T0). These findings are of therapeutic relevance in the use of phenothiazines in equine patients with inflammatory diseases where neutrophil activation and ROS production are implicated. |
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