| 我国三种野生泥鳅遗传多样性的微卫星分析 |
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| 引用本文： | 李雅娟,于卓,张明昭,庞义猛,钱聪,孙效文,荒井克俊.我国三种野生泥鳅遗传多样性的微卫星分析[J].东北农业大学学报,2010,41(12). |
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| 作者姓名： | 李雅娟 于卓 张明昭 庞义猛 钱聪 孙效文 荒井克俊 |
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| 作者单位： | 大连海洋大学生命科学与技术学院,辽宁,大连,116023;中国水产科学院黑龙江水产研究所,哈尔滨,150070;日本北海道大学大学院水产科学研究院,北海道,041-8611 |
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| 基金项目： | 日本学术振兴会论文博士奖学金项目，*，辽宁省科学技术计划重大、重点项目 |
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| 摘 要： | 本研究采用5个微卫星分子标记技术对我国黑龙江泥鳅(M.mohoity)、北方泥鳅(M.bipartitus)、泥鳅(M.anguillicaudatus)进行了遗传多样性分析。结果表明,三种泥鳅的5个微卫星基因座共检测到等位基因数27个,微卫星基因座的等位基因数范围为3~7个。5个微卫星基因座在三种泥鳅的多态信息含量都达到了高度多态的水平,平均PIC值在0.526~0.722之间,说明三种泥鳅具有丰富的遗传多样性。黑龙江泥鳅、北方泥鳅和泥鳅的观测杂合度Ho分别为0.978,0.889和0.742,期望杂合度He分别为0.683、0.781、0.721。三种泥鳅的遗传距离为0.189~0.559,泥鳅和北方泥鳅群体间遗传距离最小为0.189,亲缘关系较近,而泥鳅和黑龙江泥鳅群体间遗传距离最大为0.559,亲缘关系较远。Hardy-Weinberg平衡检验P值。得出所有个体在5个微卫星位点上都无偏离现象。聚类结果显示黑龙江泥鳅、北方泥鳅、泥鳅群体分为二支,泥鳅和北方泥鳅聚为一类,之后两者再与黑龙江泥鳅聚为一类。
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| 关 键 词： | 黑龙江泥鳅 北方泥鳅 泥鳅 微卫星 |
Genetic diversity in Chinese loach species analyzed by microsatellite DNA markers |
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| LI Yajuan,YU Zhuo,ZHANG Mingzhao,PANG Yimeng,QIAN Cong,SUN Xiaowen,KATSUTOSHI Arai.Genetic diversity in Chinese loach species analyzed by microsatellite DNA markers[J].Journal of Northeast Agricultural University,2010,41(12). |
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| Authors: | LI Yajuan YU Zhuo ZHANG Mingzhao PANG Yimeng QIAN Cong SUN Xiaowen KATSUTOSHI Arai |
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| Institution: | LI Yajuan1,YU Zhuo1,ZHANG Mingzhao1,PANG Yimeng1,QIAN Cong1,SUN Xiaowen2,KATSUTOSHI Arai3(1.Life Science and Technology Institute,Dalian Ocean University,Dalian Liaoning 116023,China,2.Heilongjiang River Fishery Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070 China,3.Graduate School of Fisheries Sciences,Hokkaido University,Hokkaido 041-8611,Japan) |
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| Abstract: | Intraspecific genetic diversity and intraspecific relationship were analyzed in loach species,Misgurnus mohoity,Misgurnus bipartitus and Misgurnus anguillicaudatus.Five loci were polymorphic in these species and a total 27 alleles were found.The number of alleles per locus ranged from 3 to 7.The loci was highly polymorphic and PIC was 0.526-0.722.Thus,these loach species had high genetic variability.In M.mohoity,M.bipartitus and M.anguillicaudatus,the observed heterozygosity(H0) was 0.978,0.889 and 0.742,an... |
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| Keywords: | Misgurnus mohoity Misgurnus bipartitus Misgurnus anguillicaudatus microsatellite analysis |
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