Enrofloxacin pharmacokinetics in Takifugu flavidus after oral administration at three salinity levels |
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| Authors: | Rongrong Ma Liu Yang Tao Ren Yaping Dong Tengfei Liu Ping Zhuang Wenhong Fang Xianle Yang Kun Hu |
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| Institution: | 1. National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China;2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Yangtze Estuary Fishery Ecology, Shanghai, China;3. Fishery and Animal Husbandry Bureau of Gaocheng District, Shijiazhuang, China |
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| Abstract: | The present study was designed to explore the pharmacokinetics of enrofloxacin in Takifugu flavidus at different salinity levels (10‰, 20‰ and 30‰). The concentrations of enrofloxacin in plasma and tissues (kidney, liver and muscle) of T. flavidus after a single oral dose of 10 mg kg?1 were simultaneously determined using HPLC. The parameters of pharmacokinetics were calculated using non‐compartmental model based on statistic moment theory. The peak concentrations of enrofloxacin were higher and Tmax values in plasma, liver and muscle tissues of T. flavidus were lower at the salinity of 20‰ and 30‰ than that of 10‰, except the kidney tissue. These demonstrated the absorption of enrofloxacin was more quickly and more quantity at the high salinity. The elimination half‐life (t1/2z) of enrofloxacin in the plasma (45.22, 69.91 and 95.45 h) was increasing with the increase in salinity. And the t1/2z values of enrofloxacin in liver (76.44, 44.21 and 33.48 h) and kidney (212.16, 157.43 and 35.61 h) both decline with the increase in salinity, indicating the elimination of enrofloxacin was faster in liver and kidney but slower in plasma at the high salinity. The content of enrofloxacin in liver was increasing with the increase in salinity, suggesting that salinity affects tissue distribution and metabolism in T. flavidus. In addition, the AUC0–∞ data of enrofloxacin (kidney > plasma > liver > muscle) at different salinity levels were essentially consistent, and AUC0–∞ data of kidney were declined with the increase in salinity, supporting that the kidney was the main organ and the role of extent of kidney on excretion was different in different salinity levels. |
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| Keywords: | enrofloxacin pharmacokinetics
Takifugu flavidus
salinity elimination |
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