| 海南番木瓜PRSV和PLDMV病毒发生情况及分子鉴定 |
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| 引用本文： | 张雨良,黄启星,郭安平,曾会才,赵辉,孔华,刘志昕.海南番木瓜PRSV和PLDMV病毒发生情况及分子鉴定[J].热带作物学报,2013,34(12):2436-2441. |
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| 作者姓名： | 张雨良 黄启星 郭安平 曾会才 赵辉 孔华 刘志昕 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室;中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室;中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室;中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室;中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室;中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室;中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室 |
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| 基金项目： | 海南省重大科技项目（No. ZDZX2013010-3）；国家自然科学基金项目（No. 31201486）；中央级公益性科研院所基本科研业务专项（No. ITBB110303）。 |
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| 摘 要： | 对海南昌江、乐东、澄迈、文昌和三亚5个主要番木瓜产区的病毒病发生情况进行调查。对76份番木瓜疑似病毒样品进行检测，检测结果表明海南番木瓜病毒病主要有2种：由番木瓜环斑病毒（Papaya ring spot virus，PRSV）引起的番木瓜环斑病毒病和由番木瓜畸形花叶病毒（Papaya leaf-distortion mosaic virus，PLDMV）引起的番木瓜畸形花叶病毒病。其中，番木瓜环斑病毒病最为常见，检出率达77.63％，是影响海南地区番木瓜产业健康发展的主要病毒病；畸形花叶病毒病发生率很低，仅检出2例番木瓜畸形花叶病毒病样品。以PRSV病毒基因组P1和Hc-Pro作为靶标基因进行分子克隆、测序，结果表明海南地区PRSV病毒可明显分为2个株系：HN-PRSV-1株系和HN-PRSV-2株系。此外，基于CP基因的系统进化树分析结果表明在海南新发现的PLDMV病毒可能源于台湾和日本。本研究可为开展番木瓜花叶病的防控和创制抗花叶病毒番木瓜新种质提供数据参考。
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| 关 键 词： | 番木瓜 PRSV PLDMV 分子鉴定 系统进化树 |
Occurrence and Distribution of Papaya ring spot virus and Papaya leaf-distortion mosaic virus in Hainan Province |
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| ZHANG Yuliang,HUANG Qixing,GUO Anping,ZENG Huicai,ZHAO Hui,KONG Hua and LIU Zhixin.Occurrence and Distribution of Papaya ring spot virus and Papaya leaf-distortion mosaic virus in Hainan Province[J].Chinese Journal of Tropical Crops,2013,34(12):2436-2441. |
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| Authors: | ZHANG Yuliang HUANG Qixing GUO Anping ZENG Huicai ZHAO Hui KONG Hua and LIU Zhixin |
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| Institution: | Institute of Tropical Bioscience and Biotechnology, CATAS / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture;Institute of Tropical Bioscience and Biotechnology, CATAS / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture;Institute of Tropical Bioscience and Biotechnology, CATAS / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture;Institute of Tropical Bioscience and Biotechnology, CATAS / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture;Institute of Tropical Bioscience and Biotechnology, CATAS / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture;Institute of Tropical Bioscience and Biotechnology, CATAS / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture;Institute of Tropical Bioscience and Biotechnology, CATAS / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture |
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| Abstract: | Papaya viral disease severely threatens papaya industry in Hainan. The occurrence and distribution of viral diseases on papaya were investigated in five producing areas in Hainan including Changjiang, Ledong, Chengmai, Wenchang and Sanya. Two major papaya viruses were presented among 76 symptomatic samples: Papaya ring spot virus(PRSV)and Papaya leaf-distortion mosaic virus(PLDMV). PRSV is a more prevalent virus(infection rate of 77.63%)and exhibits papaya yellowing, leaf distortion, severe mosaic, and even tissue necrosis. The incidence of PLDMV was rare, and only 2 samples were identified. P1 and Hc-Pro genes sequence analysis revealed that PRSV in Hainan were divided into two PRSV subgroups(HN-PRSV-1 and HN-PRSV-2). Phylogenetic analysis of CP genes revealed that the newly reported PLDMV in Hainan was likely resourced from Taiwan and Japan. The study will provide the data reference for the development of prevention and control of papaya viral diseases and the molecular breeding for virus-resistance cultivars. |
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| Keywords: | Papaya PRSV PLDMV Molecular identification Phylogenetic analysis |
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