| 酮病对荷斯坦奶牛抗氧化系统和一氧化氮含量的影响 |
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| 引用本文： | 王银龙,高树,贺志锐,刘强,赛务加甫.酮病对荷斯坦奶牛抗氧化系统和一氧化氮含量的影响[J].安徽农业科学,2013,41(15):6742-6743,6745. |
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| 作者姓名： | 王银龙 高树 贺志锐 刘强 赛务加甫 |
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| 作者单位： | 石河子大学动物科技学院,新疆石河子,832000;石河子大学动物科技学院,新疆石河子,832000;石河子大学动物科技学院,新疆石河子,832000;石河子大学动物科技学院,新疆石河子,832000;石河子大学动物科技学院,新疆石河子,832000 |
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| 摘 要： | 目的]研究新疆荷斯坦奶牛酮病血清中的部分生化指标的变化及其酮病致病机理的探讨。方法]以新疆荷斯坦奶牛为试验动物,用酮粉法和改良的水杨醛检测法对其血清进行检测,应用硝酸还原酶法测定血清中一氧化氮(NO)含量和一氧化氮合酶(NOS)活性;应用DTNB显色法测定血清中GSH-Px活力,应用黄嘌呤氧化法测定血清中T-SOD活力,应用硫代巴比妥酸法测定血清中MDA含量。结果]酮病奶牛血清中GSH-Px活力极显著降低(P0.01),T-SOD活力显著降低(P0.05),血清中MDA含量显著增高(P0.05);对照组奶牛血清中的NO含量和NOS活性均显著高于阳性组。结论]酮病不但影响奶牛的抗氧化系统而且也会降低一氧化氮含量和一氧化氮酶的活性,对于揭示奶牛酮病的发生机理具有重要意义。
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| 关 键 词： | 荷斯坦奶牛 酮病 一氧化氮 抗氧化系统 |
Effects of Ketosis on Anti-Oxidative System and NO Content of Holstein Dairy Cows |
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| Institution: | WANG Yin-long et al(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832000) |
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| Abstract: | Objective] To observe the change of biochemical indicators in serum of Xinjiang Holstein cow ketosis and disease pathogenesis.Method] Xinjiang Holstein cows were selected as experimental animals,their serum were detected with ketone powder method and improved salicylaldehyde detection method.Nitrate reductase method was used for determination of determinationserum nitric oxide（NO） content and nitric oxide synthase（NOS） activity,DTNB chromogenic assay was used to determine GSH-Px activity,the xanthine oxidation method was used for the determination of T-SOD activity,thio Pakistanthan properly acid method was adopted for determination of serum MDA content.Result] The results showed that ketosis cows serum GSH-Px activity poles（P＆lt;0.01） reduction in T-SOD activity significantly decreased（P＆lt;0.05） and serum MDA content significantly increased（P＆lt;0.05）;NO content and NOS activity in the serum of cows in the control group were significantly greater than the positive group.Conclusion] Ketosis not only affects the anti-oxidative system but also can reduce NO content and activity of NOS,which has important significance to reveal the mechanism of cow ketosis. |
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| Keywords: | Holstein dairy cows Ketosis NO Anti-oxidative system |
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