| 西瓜遗传图谱的构建及果形指数QTL 分析 |
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| 引用本文： | 王兆吉,高鹏,栾非时,刘识.西瓜遗传图谱的构建及果形指数QTL 分析[J].中国蔬菜,2013,1(14):25. |
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| 作者姓名： | 王兆吉 高鹏 栾非时 刘识 |
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| 作者单位： | （东北农业大学园艺学院，黑龙江哈尔滨 150030） |
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| 基金项目： | 国家自然科学基金项目，国家西甜瓜产业技术体系-分子育种岗位项目 |
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| 摘 要： | 以圆球形西瓜品系花园母本为母本，椭圆形西瓜品系LSW-177 为父本，配制杂交组合，构
建由180 个单株组成的F2 群体，测量成熟西瓜的果实纵径、果实横径，并且通过果实的纵径与横径的比
值得到果形指数，进行遗传图谱的构建及相关性状QTL 分析。构建的连锁遗传图谱共含有45 个SSR 标
记，分属13 个连锁群。覆盖基因组长度为547.3 cM，标记间平均距离为12.44 cM，检测到与果形指数
密切相关的QTL 1 个，并在遗传连锁图谱中将之命名为Fsi3.1，位于第三连锁群，位于标记MCPI-12 和
CMN05_69 之间，贡献率为2.8%，加性效应负值，对减小果形指数表现为增效加性效应。
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| 关 键 词： | 西瓜 果形指数 SSR 标记 QTL 分析 |
| 收稿时间： | 2013-01-24; |
Construction of Watermelon Genetic Map and Fruit Shape Index QTL Analysis |
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| WANG Zhao-ji
,
GAO Peng
,
LUAN Fei-shi
,
LIU Shi.Construction of Watermelon Genetic Map and Fruit Shape Index QTL Analysis[J].China Vegetables,2013,1(14):25. |
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| Authors: | WANG Zhao-ji GAO Peng LUAN Fei-shi LIU Shi |
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| Institution: | （Horticulture College， Northeast Agricultural University， Harbin 150030， Heilongjiang， China） |
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| Abstract: | Taking round shape watermelon line‘ Huayuanmuben’ as female parent and oval
shape line‘LSW-177’as male parent，we crossed them and obtained F2 population constructed with
180 single plants．We measured the length and width of the ripened watermelon〔Citrullus lanatus
（Thunb.）Matsum. et Nakai〕，obtained fruit shape index from the ratio between fruit length and width，
constructed the genetic map and analyzed the QTLs of relevant characteristics．The constructed genetic
map contains 45 SSR-markers belonging to 13 linkage groups．The covering genome length was 547.3
cM．The average distance between markers was 12.44 cM．The one QTL closely associated with fruit
shape index was located in the 3rd linkage group between MCPI-12 and CMN05_69．This QTL was
named as Fsi3.1．Its contribution rate was 2.8%，and its additive effect was negative，which showed
synergia additive effect in reducing fruit shape index. |
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| Keywords: | Watermelon Fruit shape index SSR marker QTL analysis |
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