| 温度和pH值对斜带髭鲷蛋白酶、淀粉酶活性的影响 |
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| 作者姓名： | 李加儿 张建强 许晓娟 区又君 陶启友 郭根喜 |
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| 作者单位： | 1. 中国水产科学研究院南海水产研究所,广州,510300
2. 中国水产科学研究院南海水产研究所,广州,510300;上海海洋大学水产与生命学院,201306 |
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| 基金项目： | 国家115科技支撑计划项目，国家高技术研究发展(863)计划项目，广东省科技计划项目 |
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| 摘 要： | 研究了温度和pH值对斜带髭鲷Hapalogenys nitens蛋白酶、淀粉酶活力的影响。结果表明，斜带髭鲷胃内pH值范围为4.9～5.4；肝胰脏pH值为5.8～6.2；肠道pH值为6.5～6.9。蛋白酶活性随温度的上升而增加，在40℃达最大，40℃以上酶活性随温度的上升而下降。在不同消化器官中，蛋白酶活性大小顺序为：胃〉前肠〉幽门盲囊〉后肠〉肝胰脏。在15～50℃范围内，斜带髭鲷消化道不同部位淀粉酶活性的最适温度均为35℃，淀粉酶活性由高到低顺序为：肝胰脏〉幽门盲囊〉前肠〉后肠〉胃。在pH值为2.2～7.6范围内，胃蛋白酶活性的最适pH值为2.8；在pH值为4.8～8.0范围内，其他消化器官蛋白酶的最适pH值均为7.2；在最适pH值下，各消化器官中的蛋白酶活性由高到低顺序为：前肠〉幽门盲囊〉后肠〉肝胰脏。在pH值为4.8～8.0范围内，胃淀粉酶活性的最适pH值为6.0，肠、肝胰脏与幽门盲囊淀粉酶的最适pH值均为6.8，活性由高到低顺序为：肝胰脏〉幽门盲囊〉前肠〉后肠〉胃。在最适温度和pH值下，蛋白酶活性由高到低顺序为：胃〉前肠〉幽门盲囊〉后肠〉肝胰脏；淀粉酶活性由高到低顺序为：肝胰脏〉幽门盲囊〉前肠〉后肠〉胃。
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| 关 键 词： | 斜带髭鲷 温度 pH值 蛋白酶 淀粉酶 活性 |
| 收稿时间： | 2008/3/14 0:00:00 |
| 修稿时间： | 2008/5/24 0:00:00 |
Effects of temperature and pH on the protease and amylase activities in skewband grunt Hapalogenys nitens |
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| Authors: | LI Jia-er ZHANG Jian-qiang XU Xiao-juan OU You-jun TAO Qi-you GUO Gen-xi |
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| Institution: | LI Jia-er, ZHANG Jian-qiang, XU Xiao-juan, OU You-jun, TAO Qi-you, GUO Gen-xi (1.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300;2.College of Fisheries and Life, Shanghai Ocean University, 201306) |
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| Abstract: | The effects of temperature and pH on protease and amylase activities in skewband grunt Hapalogenys nitens was studied. Results showed that the pH values of the stomach, hepatopancreas and intestine were 4.9-5.4,5.8-6.2;and 6.5-6.9 respectively. Protease activity rose with the increase of water temperature with a peak at 40 ℃ ,and then decreased. The order of protease activity was as follows： stomach〉foregut〉pyloric caecum〉hindgut〉hepatopancreas. The optimum temperature for amylase in H. nitens was 35 ℃ within the range of 15 -50 ℃; the order of amylase activity was as follows： hepatopancreas〉pylorie caecum〉foregut〉hindgut〉stomach. The optimum pH for protease activity in stomach was 2.8 ℃ within the range of 2.2--7.6, while the optimum pH for protease activity in other digestive organs was 7.2 ℃ with in the range of 4.8-8.0. Under the optimum pH conditions, the order of protease activity in different organs was as follows： foregut〉pyloric caecum〉hindgut〉hepatopancreas. The optimum pH for protease activity in stomach was 6.0 in the range of 4.8-8.0. The optimum pH for amylase activity in stomach was 6.0 while it was 6.8 in intestine, hepatopancreas and pyloric caecum. The order of amylase activity was as follows： hepatopancreas〉pyloric caecum〉 foregut〉hindgut〉stomach. Under the optimum temperature and pH conditions, the order of protease activity was as follows： stomach〉foregut〉pyloric caecum〉hindgut〉hepatopancreas, while that of amylase activity was. hepatopancreas〉pyloric caecum〉foregut〉hindgut〉stomach. |
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| Keywords: | Skewband grunt Hapalogenys nitens Amylase Activity Temperature pH Protease |
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